MIP-1β、IL-27、IL-35联合检测对结核性胸腔积液诊断价值分析  

Analysis of the Diagnostic Value of Combined Detection of MIP-1β、IL-27 and IL-35 in Tuberculous Pleural Effusion

作　　者：钟晓夏 周世雄 张乐 蔡维玲 Zhong Xiaoxia;Zhou Shixiong;Zhang Le;Cai Weiling(Department of Respiratory and Critical Care Medicine,Luojiang District People's Hospital of Deyang City,Deyang,Sichuan 618500,China)

机构地区：[1]德阳市罗江区人民医院呼吸与危重症医学科,四川德阳618500

出　　处：《大理大学学报》2023年第10期72-75,共4页Journal of Dali University

基　　金：德阳市科技计划重点研发项目(2021SZZ113)。

摘　　要：目的:探讨巨噬细胞炎症蛋白-1β(MIP-1β)、白细胞介素(IL)-27、IL-35联合检测对结核性胸腔积液(TPE)的诊断价值。方法:选择2021年5月至2022年12月德阳市罗江区人民医院及医疗集团医院收治的106例TPE患者为研究对象(研究组),以同期入院的106例恶性胸腔积液(MPE)患者为对照(对照组)。使用酶联免疫吸附测定法检测2组患者血清MIP-1β、IL-27、IL-35表达水平,比较2组患者入组时、研究组患者治疗前后3种细胞因子的表达水平,并绘制受试者操作特征曲线(ROC曲线)评价各指标单独检测与联合检测的诊断价值。结果:与对照组相比,研究组患者血清MIP-1β、IL-35水平显著升高,IL-27水平显著降低,差异有统计学意义(P<0.01);与研究组患者治疗前相比,研究组患者治疗后血清MIP-1β、IL-27、IL-35水平均显著降低,差异有统计学意义(P<0.01);ROC曲线分析结果显示,MIP-1β、IL-27、IL-35三者联合诊断的诊断效能显著高于各指标单独检测(P<0.05)。结论:MIP-1β、IL-27、IL-35可用于TPE与MPE的鉴别诊断,且联合检测可提高诊断效能。Objective:To explore the diagnostic value of combined detection of macrophage inflammatory protein 1β(MIP-1β)、interleukin(IL)-27 and IL-35 in tuberculous pleural effusion(TPE).Methods:A total of 106 patients with TPE admitted to Luojiang District People's Hospital and Medical Group Hospital in Deyang City from May 2021 to December 2022 were selected as the study subjects(research group),and 106 patients with malignant pleural effusion(MPE)admitted at the same time were selected as the control group.The expression levels of MIP-1β,IL-27 and IL-35 in the serum of the two groups were detected by enzyme-linked immunosorbent assay.The expression levels of the three cytokines were compared before and after treatment in the research group,and receiver operating characteristic(ROC)curve was plotted to evaluate the diagnostic value of individual and combined detection of the markers.Results:Compared to the control group,the levels of serum MIP-1βand IL-35 in the research group were significantly increased,while the level of IL-27 was significantly decreased,with statistical significance(P<0.01).Compared to before treatment,the levels of serum MIP-1β,IL-27 and IL-35 in the research group were significantly decreased after treatment,with statistical significance(P<0.01).ROC curve analysis showed that the diagnostic efficacy of the combined detection of MIP-1β,IL-27 and IL-35 was significantly higher than that of individual markers(P<0.05).Conclusion:MIP-1β,IL-27 and IL-35 can be used for the differential diagnosis of TPE and MPE,and the combined detection can improve the diagnostic efficiency.

关 键 词：巨噬细胞炎症蛋白-1β 白细胞介素-27 白细胞介素-35 结核性胸腔积液 恶性胸腔积液 联合诊断 

分 类 号：R521.7[医药卫生—内科学]