利用SRAP分子标记分析谷瘟病菌遗传多样性  

Genetic Diversity of Pyricularia oryzae Based on SRAP Molecular Markers

作　　者：刘佳[1] 张梦雅 任世龙[1] 王永芳[1] 马继芳[1] 全建章[1] 刘磊[1] 董志平[1] 白辉[1] 李志勇[1] LIU Jia;ZHANG Mengya;REN Shilong;WANG Yongfang;MA Jifang;QUAN Jianzhang;LIU Lei;DONG Zhiping;BAI Hui;LI Zhiyong(Institute of Millet Crops,Hebei Academy of Agriculture and Forestry Sciences,Key Laboratory of Minor Cereal Crops of Hebei Province,Key Laboratory of Genetic Improvement and Utilization for Featured Coarse Cereals(Co-construction by Ministry and Province),Ministry of Agriculture and Rural Affairs,Shijiazhuang 050035,China)

机构地区：[1]河北省农林科学院谷子研究所,河北省杂粮重点实验室,农业农村部特色杂粮遗传改良与利用重点实验室(部省共建),河北石家庄050035

出　　处：《华北农学报》2023年第5期179-187,共9页Acta Agriculturae Boreali-Sinica

基　　金：河北省自然科学基金(C2021301062);河北省农林科学院基本业务费(2021030201);河北省重点研发计划项目(21326338D);河北省农林科学院基本科研业务费包干制项目(HBNKY-BGZ-02);国家谷子高粱产业技术体系(CARS-06-14.5-A25)。

摘　　要：了解谷瘟病菌的变异和群体结构,为今后谷瘟病的防控及抗病品种培育提供理论基础。利用20对SRAP引物对采自9个地区的90株谷瘟病菌菌株进行了PCR扩增,利用NTSYSpc-2.11F软件进行数据分析,通过UPGMA方法进行聚类分析,使用Popgene 32软件计算各群体间的遗传多样性指数。结果表明,筛选出了8对引物用于谷瘟病菌的遗传多态性分析,这8对引物共扩增出条带1728条,其中多态性条带1492条,多态性比率为86.34%。对90株病原菌进行聚类分析,其相似性系数为0.77~0.85,遗传相似系数为0.802时,所有菌株被分为27个遗传宗谱(L1~L27),其中L1为绝对优势组群,共包含来自山东、河北、山西、河南和辽宁5个省份的29株谷瘟病菌,占总菌株的32.22%。经Popgene 32软件计算分析,9个地区谷瘟病菌的Nei′s遗传多样性指数(H)为0.1414~0.2881,Shannon′s信息指数(I)为0.1960~0.4416,河北夏谷区Nei′s遗传多样性指数和Shannon′s信息指数最高,遗传多样性最丰富,而海南群体遗传多样性最低。对不同地区谷瘟病菌群体比较,吉林群体与海南群体的遗传亲缘关系最远,而河北夏谷区群体与河北春谷区群体的亲缘关系最近。可见,不同地区谷瘟病菌的遗传多样性丰富,各菌株间存在遗传分化,但菌株间的遗传分化与地理来源无明显相关性。Understanding the variation and population structure of the foxtail millet blast provides a theoretical basis for future cultivation of resistant varieties and control of the disease.Twenty pairs of SRAP primers were used for PCR amplification of strains of P.oryzae collected from 9 regions.NTSYSpc-2.11F software was used for data analysis,UPGMA method was used for cluster analysis,and Popgene 32 software was used to calculate the genetic diversity index among populations.The results showed that 8 pairs of primers were selected with good polymorphism.A total of 1728 discernible bands were amplified by 8 pairs of primers,1492 of which showed polymorphism,and polymorphism rate was 86.34%.The cluster analysis of 90 strains of P.oryzae showed that the similarity coefficient was between 0.77-0.85.All strains were divided into 27 genetic lineages(L1-L27)when the genetic similarity coefficient was 0.802.Among them,L1 was the absolute dominant group,containing 29 strains of P.oryzae,which from Shandong,Hebei,Shanxi,Henan and Liaoning Province,accounting for 32.22%of the total strains.According to the calculation and analysis of Popgene 32 software,Nei′s genetic diversity index(H)and Shannon′s information index(I)of foxtail millet blast in 9 regions ranged from 0.1414 to 0.2881,and 0.1960 to 0.4416,respectively.Nei′s genetic diversity index and Shannon′s information index in the summer sowing region of Hebei Province were the highest,with the richest genetic diversity,while Hainan population had the lowest genetic diversity.Compared with different regions of the populations,the genetic relationship between the Jilin population and the Hainan population was the farthest,while the genetic relationship between the Hebei summer sowing millet region population and the Hebei spring sowing millet region population was the closest.It can be seen that the genetic diversity of P.oryzae in different regions was rich,and there was genetic differentiation among strains,but there was no significant correlation between genetic d

关 键 词：谷子 谷瘟病菌 SRAP 相似性系数 遗传多样性 

分 类 号：S435.651[农业科学—农业昆虫与害虫防治] S515[农业科学—植物保护]