籼稻福香占耐储藏性的蛋白质组学分析  

Analysis of the high seed storability trait in indica rice“Fuxiangzhan”

作　　者：王颖姮[1,2] 谢鸿光[1,2] 陈飞鹤 林强[1,2] 崔丽丽 吴方喜[1,2] 魏毅东[1,2] 罗曦[1,2] 陈丽萍[1,2] 蔡秋华 谢华安[1,2] 张建福[1,2] Yingheng Wang;Hongguang Xie;Feihe Chen;Qiang Lin;Lili Cui;Fangxi Wu;Yidong Wei;Xi Luo;Liping Chen;Qiuhua Cai;Huaan Xie;Jianfu Zhang(Rice Research Institute,Fujian Academy of Agricultural Sciences,Fuzhou 350019,China;Key Laboratory of Germplasm Innovation and Molecular Breeding of Hybrid Rice for South China,Ministry of Agriculture and Rural Affairs/Fuzhou Branch,National Rice Improvement Center of China/Fujian Engineering Laboratory of Crop Molecular Breeding/Fujian Key Laboratory of Rice Molecular Breeding/Incubator of National Key Laboratory of Fujian Germplasm Innovation and Molecular Breeding Between Fujian and Ministry of Sciences&Technology/Base of South China,National Key Laboratory of Hybrid Rice/National Rice Engineering Laboratory of China,Fuzhou 350003,China)

机构地区：[1]福建省农业科学院水稻研究所,福州350019 [2]农业农村部华南杂交水稻种质创新与分子育种重点实验室/福州(国家)水稻改良分中心/福建省作物分子育种工程实验室/福建省水稻分子育种重点实验室/福建省作物种质创新与分子育种省部共建国家重点实验室培育基地/杂交水稻国家重点实验室华南研究基地/水稻国家工程研究中心,福州350003

出　　处：《科学通报》2023年第28期3857-3868,共12页Chinese Science Bulletin

基　　金：福建省人民政府-中国农业科学院协同创新“5511”工程项目(XTCXGC2021001);福建省省属公益类科研院所专项(2021R1023007)资助。

摘　　要：稻谷耐储藏性对于国家粮食安全、种质资源保存及种业安全具有非常重要的意义.本研究通过人工老化处理分析福香占和航2号种子活力的变化趋势,扫描电子显微镜观察老化21 d后糙米淀粉粒结构,并对关键时间点的种胚蛋白质组进行定量分析.结果表明,人工老化后14~21 d,航2号种子活力快速下降,淀粉粒结构受到严重破坏,种子耐储藏性较差.而福香占在人工老化21 d后仍具有较强种子活力,淀粉粒结构保持良好,耐储藏性优;蛋白质组分析表明,福香占和航2号在人工老化的不同阶段,脂类、蛋白质、碳水化合物等生物合成及代谢、遗传信息加工、转录调控、营养保持、信息传递、氧化还原调节等方面均表现出差异;差异蛋白质显著富集到4个代谢通路,即亚油酸代谢,角质、木栓和蜡质的生物合成,谷胱甘肽代谢和甘油磷脂代谢.老化21 d与处理前相比较,福香占真核生物核糖体生物发生、萜类骨架生物合成、叶酸生物合成等代谢通路发生差异表达,而航2号主要为氮代谢、淀粉和蔗糖代谢及mRNA监测等途径.本研究为稻谷耐储藏性遗传机理的深入解析及培育耐储藏水稻新品种奠定了基础.As the duration of rice seed storage increases,the storability of the rice deteriorates,with the seed losing its vigor,especially under conditions of high temperature and high humidity,as occurs in the summer in southern China.The maintenance of rice seed longevity(“shelf life”)during storage is central not only to national food security but also to the conservation of germplasm resources and the security of the seed industry.In the current study,the characteristics of the impacts of artificially accelerated aging stress on seed vigor in two indica rice cultivars,namely the high-storability“Fuxiangzhan”and low-storability“Hang2”,were compared.Between 14 and 21 days of artificial aging,the seed vigor of“Hang2”decreased rapidly and the starch grain structure,observed by scanning electron microscopy,showed serious damage,whereas seeds of“Fuxiangzhan”still exhibited strong seed vigor and good starch grain structure after 21 days of artificial aging.Quantitative analysis of the seed embryo proteome showed that the biosynthesis and metabolism of lipids,proteins and carbohydrates,genetic information processing,transcriptional regulation,nutrient retention,signal transduction and redox regulation showed marked differences between“Fuxiangzhan”and“Hang2”at various time points during artificial aging.Differentially expressed proteins between Fuxiangzhan and Hang2 were significantly enriched in four metabolic pathways:linoleic acid metabolism;cutin,suberin and wax biosynthesis;glutathione metabolism;and glycerophospholipid metabolism.We speculate that the possible reasons for the longer shelf life of“Fuxiangzhan”seeds were that the lipid oxidase activity and the linoleic acid peroxidation rate of the seed embryos were lower than in“Hang2”,and that the seeds had an improved protective barrier associated with greater concentrations of cutin and other waxes.Under high temperature and humidity stresses,the glutathione antioxidant system played a role in rapidly scavenging reactive oxygen speci

关 键 词：福香占 稻谷耐储藏性 种子活力 脂肪氧化酶 蛋白质组 

分 类 号：S511.21[农业科学—作物学]