A paternal protein facilitates sperm RNA delivery to regulate zygotic development  

作　　者：Dongdong Li Shijing Huang Yongping Chai Ruiqian Zhao Jing Gong Qiangfeng Cliff Zhang Guangshuo Ou Wenyu Wen 

机构地区：[1]Tsinghua-Peking Center for Life Sciences,Beijing Frontier Research Center for Biological Structure,McGovern Institute for Brain Research,School of Life Sciences and MOE Key Laboratory for Protein Science,Tsinghua University,Beijing 100084,China [2]Department of Neurosurgery,Huashan Hospital,the Shanghai Key Laboratory of Medical Epigenetics,Institutes of Biomedical Sciences,State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science,National Center for Neurological Disorders,School of Basic Medical Sciences,Fudan University,Shanghai 200032,China

出　　处：《Science China(Life Sciences)》2023年第10期2342-2353,共12页中国科学（生命科学英文版）

基　　金：the Ministry of Science and Technology of China(2019YFA0508401);the National Natural Science Foundation of China(31871394,82121004,32100538);Shanghai Municipal Science and Technology Major Project(2018SHZDZX01);the China Postdoctoral Science Foundation(2022M711844);the Young Elite Scientists Sponsorship Program by CAST(YESS20220102);ZJ Lab and Shanghai Center for Brain Science and Brain-Inspired Technology。

摘　　要：Sperm contributes essential paternal factors,including the paternal genome,centrosome,and oocyte-activation signals,to sexual reproduction.However,it remains unresolved how sperm contributes its RNA molecules to regulate early embryonic development.Here,we show that the Caenorhabditis elegans paternal protein SPE-11 assembles into granules during meiotic divisions of spermatogenesis and later matures into a perinuclear structure where sperm RNAs localize.We reconstitute an SPE-11 liquid-phase scaffold in vitro and find that SPE-11 condensates incorporate the nematode RNA,which,in turn,promotes SPE-11 phase separation.Loss of SPE-11 does not affect sperm motility or fertilization but causes pleiotropic development defects in early embryos,and spe-11 mutant males reduce m RNA levels of genes crucial for an oocyte-to-embryo transition or embryonic development.These results reveal that SPE-11 undergoes phase separation and associates with sperm RNAs that are delivered to oocytes during fertilization,providing insights into how a paternal protein regulates early embryonic development.

关 键 词：SPE-11 phase separation paternal factor early embryonic development 

分 类 号：Q132[生物学—普通生物学]