中华绒螯蟹幼蟹碳稳定同位素周转速率与碳、氮分馏系数的测定  

Determination of carbon stable isotope turnover rate and carbon and nitrogen fractionation factor in juvenile Eriocheir sinensis

作　　者：鹿珍珍 徐广伟 孙云飞 肖昌伦 唐伯平[5] 成永旭[1,2,3,4] LU Zhenzhen;XU Guangwei;SUN Yunfei;XIAO Changlun;TANG Boping;CHENG Yongxu(Centre for Research on Environmental Ecology and Fish Nutrion,Ministry of Agriculture and Rural 4ffairs,Shanghai Ocean University,Shanghai 201306,China;Key Laboratory of Integrated Rice-Fish Farming Ecology,Minstry of Agriculure and Rural Affairs,Fresh water Fisheries Research Center,Chinese Academy of Fishery Sciences,Wixi 214081,China;National Demonstration Center for Experimental Fisheries Science Educution,Shanghai Ocean University,Shanghai 201306,China;Shanghai Engineering Research Center of Aquaculnure,Shanghai Ocean Universiy,Shanghai 201306,China;Yancheng Teachers University,Yancheng 224002,China)

机构地区：[1]上海海洋大学,农业农村部鱼类营养和环境生态研究中心,上海201306 [2]中国水产科学研究院淡水渔业研究中心,农业农村部稻渔综合种养生态重点实验室,江苏无锡214081 [3]上海海洋大学,水产科学国家级实验教学示范中心,上海201306 [4]上海海洋大学,上海水产养殖工程中心,上海201306 [5]盐城师范学院,江苏盐城224002

出　　处：《水产学报》2023年第10期126-133,共8页Journal of Fisheries of China

基　　金：现代农业产业技术体系专项(CARS-48);国家自然科学基金青年基金(31802320)。

摘　　要：为测定生长与新陈代谢对不同规格中华绒螯蟹幼蟹和不同组织碳稳定同位素周转速率的影响及分馏系数,本实验在水族箱(0.2 m×0.2 m×0.3 m)中分别养殖EA组[(4.0 g±0.5) g]和EB组[(15.5 g±0.5) g]2组中华绒螯蟹幼蟹,分别于0、7、14、21和28 d时采集幼蟹的肝胰腺、肌肉和鳃组织,探究其碳稳定同位素周转过程,并测定了幼蟹对食物碳、氮稳定同位素的分馏系数。结果显示,EA组幼蟹碳稳定同位素的周转速率大于EB组,周转速率随着幼蟹规格的增大而减小。幼蟹肝胰腺的周转速率最快,其次是肌肉,最后是鳃,其中EA组幼蟹肝胰腺、肌肉和鳃的半衰期分别为20.88、29.37和34.14 d,EB组分别为21.73、35.18和66.01 d。新陈代谢是驱动幼蟹体内碳稳定同位素周转的主要因子,幼蟹肝胰腺、鳃和肌肉中新陈代谢占碳稳定同位素周转的贡献百分比分别为79.82%~94.04%、67.00%~81.90%和71.61%~90.36%。幼蟹对饵料碳、氮稳定同位素的分馏系数分别为0.98‰±0.19‰和3.01‰±0.02‰。本研究不仅对中华绒螯蟹幼蟹的营养生态学研究提供参考,还为中华绒螯蟹养殖过程中的饵料选择提供了科学依据。To determine the effects of growth and metabolism on carbon stable isotope turnover rates and fractionation factor of juvenile Eriocheir sinensis and different tissues,the hepatopancreas,muscle and gill tissues of juvenile E.sinensis in EA group[(4.0 g±0.5)g]and EB group[(15.5 g±0.5)g]were collected at 0,7,14,21 and 28 days,respectively,in an aquarium of 0.2×0.2×0.3 m.The turnover process of stable carbon isotopes was investigated,and the fractionation factor ofstable carbon and nitrogen isotopes were determined.Theresults showed that(1)The turnover rate of carbon stable isotopes in EA group was greater than that in EB group,and the turnover rate decreased with the increase of juvenile crab size.(2)The turnover rate of hepatopancreas was the fastest,followed by muscle and gills.The half-lives of hepatopancreas,muscle and gills in EA group were 20.88,29.37 and 34.14 days,respectively,and those in EB group were 21.73,35.18 and 66.01 days,respectively.(3)Metabolism was more principal for driving the turnover of carbon stable isotope with the contribution ranging from 79.82%to 94.04%for hepatopancreas,from 67.00%to 81.90%for gills,and from 71.61%to 90.36%for muscle.(4)The fractionation factors of carbon and nitrogen stable isotopes were 0.98%o±0.19%o and 3.01%o±0.02%o,respectively.This study not only provided a reference for the nutritional ecology of juvenile E.sinensis,but also provided a scientific basis for bait selection in the breeding process of E.sinensis.

关 键 词：中华绒螯蟹 碳周转速率 生长 新陈代谢 分馏系数 稳定同位素 

分 类 号：S966.16[农业科学—水产养殖]