Recombineering enables genome mining of novel siderophores in a non-model Burkholderiales strain  

作　　者：Xingyan Wang Haibo Zhou Xiangmei Ren Hanna Chen Lin Zhong Xianping Bai Xiaoying Bian 

机构地区：[1]Helmholtz International Lab for Anti-Infectives,State Key Laboratory of Microbial Technology,Shandong University-Helmholtz Institute of Biotechnology,Shandong University,Qingdao 266237,China

出　　处：《Engineering Microbiology》2023年第3期30-36,共7页工程微生物学（英文）

基　　金：supported by the National Key R&D Program of China(2019YFA0905700);National Natural Science Foundation of China(32070060).

摘　　要：Iron is essential for bacterial survival,and most bacteria capture iron by producing siderophores.Burkholde-riales bacteria produce various types of bioactive secondary metabolites,such as ornibactin and malleobactin siderophores.In this study,the genome analysis of Burkholderiales genomes showed a putative novel siderophore gene cluster crb,which is highly similar to the ornibactin and malleobactin gene clusters but does not have pvdF,a gene encoding a formyltransferase for N-δ-hydroxy-ornithine formylation.Establishing the bacteriophage recom-binase Redγ-Redδβ7029 mediated genome editing system in a non-model Burkholderiales strain Paraburkholderia caribensis CICC 10960 allowed the rapid identification of the products of crb gene cluster,caribactins A-F(1-6).Caribactins contain a special amino acid residue N-δ-hydroxy-N-δ-acetylornithine(haOrn),which differs from the counterpart N-δ-hydroxy-N-δ-formylornithine(hOrn)in ornibactin and malleobactin,owing to the absence of pvdF.Gene inactivation showed that the acetylation of hOrn is catalyzed by CrbK,whose homologs proba-bly not be involved in the biosynthesis of ornibactin and malleobactin,showing possible evolutionary clues of these siderophore biosynthetic pathways from different genera.Caribactins promote biofilm production and en-hance swarming and swimming abilities,suggesting that they may play crucial roles in biofilm formation.This study also revealed that recombineering has the capability to mine novel secondary metabolites from non-model Burkholderiales species.

关 键 词：Burkholderiales Recombineering Siderophore Genome mining Caribactins 

分 类 号：Q93[生物学—微生物学]