珍珠梅组培快繁体系的建立  

Establishment of tissue culture rapid propagation system of Sorbaria sorbifolia

作　　者：易元慧 刘艳军 许丁帆 冉静 Yi Yuanhui;Liu Yanjun;Xu Dingfan;Ran Jing(College of Horticulture and Landscape,Tianjin Agricultural University,Tianjin 300392,China)

机构地区：[1]天津农学院园艺园林学院,天津300392

出　　处：《天津农学院学报》2023年第4期8-11,共4页Journal of Tianjin Agricultural University

基　　金：天津市科技计划项目(18ZXBFNC00370);河北省产业创新创业团队项目(199A2905H);河北省创新能力提升计划项目(20566301D);河北省中央引导地方科技发展资金项目(206Z6303G)。

摘　　要：以珍珠梅茎段为外植体,研究不同消毒时间、不同培养基、激素种类和浓度对珍珠梅离体培养的影响,建立了珍珠梅组培快繁体系,结果发现:用2%有效氯次氯酸钠,消毒8 min,接种茎段成活率最理想;使用培养基WPM+GA35 mg/L作为初代培养,腋芽萌发率极高,平均生长量长为6.01 cm,生长效果最为理想;培养基MS+BA 0.5 mg/L+IAA 1.0 mg/L作为继代增殖培养基,培养30 d后增殖系数是8.13,效果最佳;生根培养基条件效果最佳为1/2 MS+NAA 0.5 mg/L;试验中将V_(草炭)∶V_(蛭石)∶V_(珍珠岩)=1∶1∶1作为移栽基质,可达到理想的移栽效果,在保证根系完整的情况下,30 d后调查移栽成活率可达到90%以上。In order to establish a rapid propagation system for tissue culture of Sorbaria sorbifolia,in this study the stem segments of Sorbaria sorbifolia were used as explants to study the effects of different disinfection times,different basic media,hormone types and concentrations on in vitro culture of Sorbaria sorbifolia.The results showed that using a 2%effective chlorine sodium hypochlorite solution for disinfection for 8 minutes and inoculating the stem segment disinfectant had the most ideal effect;using WPM+GA35 mg/L as the primary culture medium,the germination rate of axillary buds was the highest,with an average growth length of 6.01 cm,and the growth effect was the most ideal;using MS+BA 0.5 mg/L+IAA 1.0 mg/L as the proliferation medium,the proliferation coefficient reached 8.13 after 30 days of cultivation,indicating the best effect;the optimal rooting medium is 1/2 MS+NAA 0.5 mg/L;using peat∶vermiculite∶perlite=1∶1∶1(v/v)as the transplant substrate,the survival rate of transplantation can reach over 90%after 30 days,while ensuring the integrity of the root system.

关 键 词：珍珠梅 快繁 茎段 腋芽 

分 类 号：S685[农业科学—观赏园艺]