HPLC-ELSD法和UPLC-MS/MS法评价石斛夜光丸中人参投料掺伪情况  

Evaluation of Ginseng Radix et Rhizoma in Shihu Yeguang pills by HPLC-ELSD and UPLC-MS/MS

作　　者：朱韬 何颂华 李丽莉 罗轶 ZHU Tao;HE Song-hua;LI Li-li;LUO Yi(Guangxi Institute for Food and Drug Control,NMPA Key Laboratory for Quality Monitor and Evaluation of Traditional Chinese Medicine,Chinese Material Medica,Nanning 530021)

机构地区：[1]广西壮族自治区食品药品检验所国家药品监督管理局中药材质量监测与评价重点实验室,南宁530021

出　　处：《中南药学》2023年第10期2729-2733,共5页Central South Pharmacy

摘　　要：目的建立石斛夜光丸中人参混淆品(西洋参)掺伪的检测方法,考察样品中人参的投料情况。方法分别采用HPLC-ELSD和UPLC-MS/MS法进行分析测定。HPLC-ELSD法:采用CAPCELL PAK C18 MGⅢ(S-5)(4.6 mm×250 mm,5μm)柱,以乙腈-水为流动相,流速为1.0 mL·min^(-1),柱温30℃,ELSD漂移管温度为105℃,气体流速为3.0 L·min^(-1);UPLC-MS/MS法:采用电喷雾离子源(ESI-),多反应监测(MRM)模式。结果91批石斛夜光丸中有35批样品检出西洋参。结论所建立方法专属性、重复性、稳定性、耐用性良好,可为石斛夜光丸中人参的质量控制与评价提供可靠方法。Objective To detect adulterated Ginseng Radix et Rhizoma in Shihu Yeguang pills.Methods The samples were determined by HPLC-ELSD and UPLC-MS/MS.The chromatographic separation was performed on a CAPCELL PAK C18 MGⅢ(S-5)(4.6 mm×250 mm,5μm)column with the mobile phase consisting of acetonitrile-water at 1.0 mL·min^(-1) in a gradient elution.The columm temperature was 30℃,the temperature of ELSD was 105℃,and the gas flow rate was 3.0 L·min^(-1).The electrospray was operated in the negative mode,and the samples were detected by multiple reaction monitoring mode(MRM).Results Among the 91 batches of Shihu Yeguang pills,35 samples batches were adulterated with Panacis Quinquefolii Radix.Conclusion The methods shows good specificity,repeatability,stability and durability,which is reliable for the quality control and evaluation of Ginseng Radix et Rhizoma in Shihu Yeguang pills.

关 键 词：石斛夜光丸 HPLC-ELSD UPLC-MS/MS 人参 西洋参 质量控制 

分 类 号：R284.1[医药卫生—中药学]