RNA剪接中的SF3B1功能及其磷酸化修饰的研究进展  

Advances in the Functional Role of SF3B1 in RNA Splicing and its Phosphorylation Modifications

作　　者：刘畅晓风 方秋月 谢微嫣 LIU Chang-Xiao-Feng;FANG Qiu-Yue;XIE Wei-Yan(Department of Cell Biology,Beijing Neurosurgical Institute,Capital Medical University,Beijing 100070,China)

机构地区：[1]首都医科大学北京市神经外科研究所细胞生物室,北京100070

出　　处：《生理科学进展》2023年第5期351-358,共8页Progress in Physiological Sciences

基　　金：国家自然科学基金(82071559,82071558)资助课题。

摘　　要：RNA剪接作为真核生物基因表达的关键环节,通过去除前体mRNA中的内含子并将外显子连接,对基因表达调控起到了至关重要的作用。此过程主要由剪接体复合物介导,而剪接体的组装和激活则主要依赖剪接因子的促进,从而协助完成两个步骤的酯交换。剪接因子3B亚基1(splicing factor 3B subunit 1,SF3B1)是组成剪接体复合物的U2 RNP的关键部分,其磷酸化修饰水平改变细胞剪接过程,进而对正常细胞功能或肿瘤细胞的增殖、迁移等产生影响。本文总结了迄今为止磷酸化蛋白组学中关于SF3B1的部分,重点关注SF3B1在剪接中的功能以及磷酸化修饰对其功能影响的研究进展。RNA splicing,a critical process in eukaryotic gene expression,plays a fundamental role in the regulation of gene expression by removing introns from precursor mRNA and joining exons.This process is primarily mediated by the spliceosome complex,while the assembly and activation of the spliceosome predominantly rely on splicing factors,facilitating the two-step transesterification.The splicing factor 3B subunit 1(SF3B1)occupies a crucial position within the U2 RNP that constitutes the spliceosome complex.Alternations in the phosphorylation level of SF3B1 affect the cellular splicing process,subsequently influencing normal cell function,as well as the proliferation and migration of tumor cells.This article summarizes the research to date on SF3B1 within the context of phosphoproteomics,with particular emphasis on the role of SF3B1 in splicing and the impact of phosphorylation modifications on SF3B1.

关 键 词：RNA剪接 剪接体 SF3B1 SAP155 磷酸化 

分 类 号：Q522[生物学—生物化学] R34[医药卫生—基础医学]