Micro-CT分析跟骨内骨小梁分布及结构特征  

Micro-CT analysis of distribution and structural characteristics of bone trabeculae in the calcaneus

作　　者：张凤珍 孙瑞芬[2] 李梓瑜 王星[3] 李琨 李志军 张少杰 Zhang Fengzhen;Sun Ruifen;Li Ziyu;Wang Xing;Li Kun;Li Zhijun;Zhang Shaojie(School of Basic Medicine,Inner Mongolia Medical University,Hohhot 010110,Inner Mongolia Autonomous Region,China;Second Affiliated Hospital of Inner Mongolia Medical University,Hohhot 010110,Inner Mongolia Autonomous Region,China;Digital Medicine Center,Inner Mongolia Medical University,Hohhot 010110,Inner Mongolia Autonomous Region,China)

机构地区：[1]内蒙古医科大学基础医学院,内蒙古自治区呼和浩特市010110 [2]内蒙古医科大学第二附属医院,内蒙古自治区呼和浩特市010110 [3]内蒙古医科大学数字医学中心,内蒙古自治区呼和浩特市010110

出　　处：《中国组织工程研究》2024年第24期3885-3889,共5页Chinese Journal of Tissue Engineering Research

基　　金：内蒙古自治区教育厅科技领军人才和创新团队建设项目(NMGIRT2307),项目负责人:张少杰;内蒙古医科大学校级重点项目(YKD202ZD007),项目负责人:张少杰;内蒙古医科大学青年领创团队项目(QNLC-2020025),项目负责人:张少杰;内蒙古自治区蒙医药协调创新中心科研项目(MYYXTYB202101),项目负责人:张少杰;内蒙古自治区卫生健康科技计划项目(202201219),项目负责人:张少杰;国家自然科学基金(81660358),项目负责人:张少杰;国家自然科学基金(81860382),项目负责人:王星;国家自然科学基金(81860383),项目负责人:李志军;内蒙古自治区自然科学基金资助项目(2019MS08017),项目负责人:张少杰;内蒙古自治区自然科学基金资助项目(2020MS03061),项目负责人:王星;内蒙古自治区自然科学基金资助项目(2020LH08021),项目负责人:李志军。

摘　　要：背景:跟骨位于足的后下部,受力较大。跟骨外形复杂且不规则,外周有较薄的皮质骨包绕,内部充满大量的骨小梁,探讨骨小梁的微观结构、走行及分布特征有助于提高对跟骨骨折的认识。目的:采用微计算机断层扫描仪(Micro-CT)扫描跟骨标本获得其影像资料,对其内部骨小梁结构进行分析,探讨跟骨内骨小梁的形态、分布及结构特征。方法:采用Micro-CT连续扫描干燥成人跟骨标本,扫描后获得影像图像,将其以DICOM格式存储。导入影像资料到Hiscan Analyzer软件,显示清晰完整的成人跟骨矢状面、冠状面、水平面的图像,逐层观察骨小梁的走行,依据骨小梁走行特点将跟骨矢状面分为6个部分,在以7 mm为标准的相同厚度下,每个部分选择1个49 mm2等面积的兴趣区,三维重建后获得跟骨及骨小梁的立体微观结构,二值化后运用软件计算感兴趣区域内骨小梁的体积分数、表面密度、骨小梁厚度、骨小梁间隙和骨小梁数量参数。结果与结论:①跟骨表面皮质层很薄,内部充满大量骨松质,在Gissane角的骨皮质明显增厚;②跟骨上部的骨小梁体积分数大于跟骨下部前端、中和三角区、跟骨下后部、跟骨结节部、跟骨底部,跟骨结节部的骨小梁体积分数大于跟骨下部前端、中和三角区、跟骨下后部、跟骨底部;跟骨结节部的骨小梁表面密度大于跟骨下部前端、中和三角区、跟骨底部,跟骨上部、跟骨下后部的骨小梁表面密度大于中和三角区;跟骨上部的骨小梁厚度大于跟骨结节部;中和三角区的骨小梁间隙大于跟骨上部、跟骨结节部;跟骨结节部的骨小梁数量大于中和三角区;③中和三角区主要分布杆状骨小梁,骨小梁表面密度最小、体积分数较小、骨小梁间隙最大;该部位骨质较疏松,受到高冲击力时抗压缩能力差,骨小梁最先发生断裂,是易发生骨折的部位。BACKGROUND:The calcaneus is located in the lower posterior part of the foot and is heavily stressed.Calcaneus is complex and irregular in shape,surrounded by thin cortical bone and filled with a large number of trabeculae.The study of the microstructure,movement,and distribution of trabeculae is helpful to improve the understanding of calcaneus fracture.OBJECTIVE:The image data of calcaneus were obtained by scanning the calcaneus specimens with micro-computed tomography,and the structure of trabecular bone in calcaneus was analyzed to explore the morphology,distribution and structural characteristics of trabecular bone in calcaneus.METHODS:Dry adult calcaneus specimens were continuously scanned by micro-computed tomography,and the images were obtained after scanning and stored in DICOM format.Image data were imported into Hiscan Analyzer software to display clear and complete images of the sagittal plane,coronal plane,and the horizontal plane of the adult calcaneus.The trabecular movement of bone was observed layer by layer.According to the trabecular movement characteristics,the sagittal plane of the calcaneus was divided into six parts.A 49-mm2 region of interest was selected for each part at the same thickness as 7 mm.The three-dimensional microstructure of calcaneus and trabecular bone was obtained after three-dimensional reconstruction.After binarization,the volume fraction,surface density,trabecular thickness,trabecular space,and trabecular number parameters of the trabecular bone in the region of interest were calculated by software.RESULTS AND CONCLUSION:(1)The cortical layer of the calcaneus was very thin and filled with a large amount of cancellous bone,and the cortical layer of the horn of Gissane was obviously thickened.(2)The trabecular volume fraction in the upper part of the calcaneus was greater than that in the anterior part of the lower part of the calcaneus,the central triangle,the posterior part of the lower part of the calcaneus,and the base of the calcaneus,and the trabecular volume fraction

关 键 词：MICROCT 跟骨 骨小梁 形态特征 骨折 

分 类 号：R445[医药卫生—影像医学与核医学] R323[医药卫生—诊断学] R681.8[医药卫生—临床医学]