变应性鼻炎儿童蒿花粉变应原组分诊断  

Component-resolved diagnosis of artemisia pollen in children with allergic rhinitis

作　　者：王育民 宋惠芳 赵晨 夏远 张星光 WANG Yumin;SONG Huifang;ZHAO Chen;XIA Yuan;ZHANG Xingguang(Department of Pediatrics,Inner Mongolia Maternal and Child Health Hospital,Hohhot 010000,China;Graduate School of Inner Mongolia Medical University,Hohhot 010010,China;School of Public Health,Inner Mongolia Medical University,Hohhot 010010,China)

机构地区：[1]内蒙古自治区妇幼保健院儿内科,内蒙古呼和浩特010000 [2]内蒙古医科大学研究生学院,内蒙古呼和浩特010010 [3]内蒙古医科大学公共卫生学院,内蒙古呼和浩特010010

出　　处：《中国耳鼻咽喉颅底外科杂志》2023年第5期46-50,共5页Chinese Journal of Otorhinolaryngology-skull Base Surgery

基　　金：内蒙古自治区自然科学基金(2020MS08200)。

摘　　要：目的本文旨在对季节性变应性鼻炎(AR)患儿进行变应原组分筛查,了解AR患儿代表性蒿属植物花粉变应原致敏组分。方法采集黄花蒿和大籽蒿花粉,提取花粉蛋白,以临床确诊为AR且艾蒿特异性免疫球蛋白E(sIgE)检测为阳性患儿血清为一抗,通过蛋白质免疫印迹法筛查花粉中变应原组分。结果蛋白质免疫印迹显示2种蒿属植物花粉蛋白提取物中主要有3条带能与血清IgE特异性结合,相对分子量(Mr)分别为12、25和70 kDa。黄花蒿花粉中70 kDa变应原为AR儿童最常见的变应原分子,阳性检出率为85.19%,25 kDa和12 kDa变应原分别为37.04%和14.81%。大籽蒿花粉中70 kDa变应原阳性检出率低于黄花蒿(P<0.05),为51.85%,25 kDa和12 kDa变应原阳性检出率与黄花蒿相似(P>0.05)。另外,同一个体血清对2种蒿属植物同源变应原阳性反应存在差异,不同患儿对1种或多种变应原分子敏感。结论AR患儿黄花蒿花粉致敏率更高,推荐增加黄花蒿花粉组分蛋白进行诊断和免疫治疗,尤其是70 kDa变应原分子。Objective To screen children with seasonal allergic rhinitis(AR)for allergen components and understand the allergenic components of pollen in children with AR.Methods Artemisia annua and artemisia sieversiana pollens were collected and pollen proteins were extracted.Taking serum from children with clinically confirmed AR and special immunoglobulin E(sIgE)positive for artemisia as primary antibody,western blotting was used to screen for allergenic components in pollen.Results Western blotting showed that three main bands of the protein extracts were specifically bound to sIgE,with relative molecular mass(Mr)of 12,25,and 70 kDa,respectively.The 70 kDa allergen in Artemisia annua pollen was the most common allergen molecule in children with AR,with a positive detection rate of 85.19%,25 kDa and 12 kDa allergens of 37.04%and 14.81%,respectively.The positive detection rate of 70 kDa allergen in Artemisia sieversiana was lower than that of Artemisia annua 51.85%(P<0.05),and the positive detection rates of 25 kDa and 12 kDa allergens were similar to those of Artemisia scutellaria(P>0.05).In addition,there were differences in the positive responses of the same serum to the two artemisia homologous allergens,and different children were sensitive to one or more allergen molecules.Conclusion With high sensitization rate of Artemisia annua pollen in AR children,it is recommended to increase the pollen component protein of Artemisia scutellaria,especially the 70 kDa allergen molecule,for the diagnosis and immunotherapy of pediatic AR.

关 键 词：变应性鼻炎 儿童 蒿属植物花粉 变应原 

分 类 号：R765.21[医药卫生—耳鼻咽喉科]