慢性间歇性低氧对大鼠肠道菌群与血糖的影响研究  

Effects of chronic intermittent hypoxia on intestinal flora and blood sugar in rats

作　　者：李雯[1,2] 刘娅钦 路苹 万自芬[1,2] 张湘燕 赵丹[1,2] LI Wen;LIU Yaqin;LU Ping;WAN Zifen;ZHANG Xiangyan;ZHAO Dan(Department of Respiratory and Critical Care Medicine,Guizhou Provincial People’s Hospital,Guiyang,Guizhou 550002,China;Key Laboratory of Pulmonary Immune Diseases,National Health Commission,Guiyang,Guizhou 550000,China)

机构地区：[1]贵州省人民医院呼吸与危重症医学科,贵阳550002 [2]国家卫生健康委员会肺脏免疫性疾病重点实验室,贵阳550000

出　　处：《重庆医学》2023年第20期3066-3070,3076,共6页Chongqing medicine

基　　金：贵州省基础研究计划项目(黔科合基础[2020]1Y291,黔科合基础-ZK[2023]一般202)。

摘　　要：目的探讨慢性间歇性低氧(CIH)对大鼠肠道菌群失调与血糖变化的影响。方法选取30只健康雄性SD大鼠,采用随机数字表法分为正常对照组(NC组)、CIH 4周组(CIH4组)、CIH 8周组(CIH8组),每组10只。分析大鼠肠道菌群种类及丰度表达,检测静脉血中肿瘤坏死因子α(TNF-α)、脂多糖(LPS)、胰岛素(INS)、空腹血糖(FBG)水平,计算胰岛素抵抗指数(HOMA-IR),观察胰腺及肠道组织病理学改变及核因子-κB(NF-κB)表达。结果CIH4组、CIH8组大鼠FBG、LPS、HOMA-IR、TNF-α水平均高于NC组(P<0.05),且CIH8组变化更明显(P<0.05)。CIH8组大鼠INS水平均高于NC组、CIH4组(P<0.05),但NC组与CIH4组比较差异无统计学意义(P>0.05)。CIH可导致肠道组织广泛黏膜糜烂,上皮细胞脱落,固有层广泛可见较多炎性细胞浸润;胰腺组织腺体杯状细胞消失,有的地方可见腺体脱落,残留基底组织。CIH8组大鼠胰腺和肠道组织中NF-κBp65表达高于NC组(P<0.05),其余组间比较差异均无统计学意义(P>0.05)。结论CIH暴露可使大鼠肠道菌群失调,血糖水平改变。Objective To investigate the effects of chronic intermittent hypoxia(CIH)on intestinal flora imbalance and blood sugar changes in rats.Methods A total of 30 healthy male Sprague-Dawley rats were randomly divided into the normal control group(NC group),the CIH4 weeks group(CIH4 group),and the CIH8 weeks group(CIH8 group),with 10 rats in each group.The types and abundance of intestinal flora in rats were analyzed.The levels of tumor necrosis factor-α(TNF-α),lipopolysaccharide(LPS),insulin(INS)and fasting blood glucose(FBG)in venous blood were detected.The insulin resistance index(HOMA-IR)was calculated.The histopathological changes of pancreas and intestine and the expression of nuclear factor of Kappa B(NF-κB)were observed.Results The levels of FBG,LPS,HOMA-IR,and TNF-α in the CIH4 and CIH8 groups were significantly higher than those in the NC group(P<0.05),and the changes in the CIH8 group were more obvious(P<0.05).The INS levels of rats in the CIH8 group were higher than those in the NC group and CIH4 group(P<0.05),but there was no significant difference between the NC group and the CIH4 group(P>0.05).CIH can lead to extensive mucosal erosion of intestinal tissue,epithelial cell shedding,and more inflammatory cells infiltration in the lamina propria;The goblet cells of pancreatic gland disappeared,and glands fell off in some places,leaving basal tissue.The expression of NF-κBp65 in pancreas and intestine of rats in the CIH8 group was higher than that in the NC group(P<0.05),but there was no significant difference among other groups(P>0.05).Conclusion CIH exposure can lead to imbalance of intestinal flora and changes of blood sugar level in rats.

关 键 词：间歇性低氧 肠道菌群紊乱 炎症反应 胰岛素抵抗指数 

分 类 号：R56[医药卫生—呼吸系统]