橡皮生肌膏对实验猪创面模型愈合的影响及创面模型制备动物品种选择  

Effect of Xiangpishengji Ointment on wound healing of experimental pig and consideration on animal breed selection for wound model development

作　　者：郭晶[1] 孙巍巍 王一鸣 戴睿 朱朝军[3] 孙旭 张朝晖[3] GUO Jing;SUN Wei-wei;WANG Yi-ming;DAI Rui;ZHU Chao-jun;SUN Xu;ZHANG Zhao-hui(Tianjin University of Chinese Medicine,Tianjin 300163,China;Tianjin Rehabilitation and Nursing Center,Tianjin 300100,China;The Second Affiliated Hospital of Tianjin University of Chinese Medicine,Tianjin 300250,China)

机构地区：[1]天津中医药大学,天津300163 [2]天津康复疗养中心,天津300100 [3]天津中医药大学第二附属医院,天津300250

出　　处：《中国新药杂志》2023年第18期1899-1904,共6页Chinese Journal of New Drugs

基　　金：国家重点研发计划资助项目(2019YFC1709303)。

摘　　要：目的:观察橡皮生肌膏对小型巴马香猪创面模型的影响,初步观察橡皮生肌膏对实验猪创面中VEGF-Notch信号通路的影响,比较实验猪和啮齿类动物创面愈合的区别,探讨创面模型制备选择实验猪的合理性。方法:在4只小型巴马香猪背部共制备48个疮面,分为2组,分别为康惠尔水凝胶组和橡皮生肌膏组,术后d 0,d 7,d 14进行拍照,使用Image J软件测量创面面积,计算创面愈合率,记录创面愈合时间,HE染色观察病理变化情况,免疫组织化学法检测创面组织中VEGF-Notch信号通路相关因子的表达情况。结果:橡皮生肌膏能够促进实验猪的创面愈合、提高创面愈合率、缩短愈合时间。橡皮生肌膏能够促进VEGF-Notch信号通路相关因子的表达。结论:橡皮生肌膏能够促进小型巴马香猪创面模型的愈合,可能与影响VEGF-Notch信号通路有关,实验猪在生理和病理方面更趋近于人类,未来可能成为创面模型制备的精准模型。Objective:To observe the effect of Xiangpishengji Ointment on the wound surface model of small Bama pigs,preliminarily observe the effect of Xiangpishengji Ointment on the VEGF-Notch signaling pathway in the wound surface of experimental pigs,compare the difference in wound healing between experimental pigs and rodents,and explore the rationality of selecting experimental pigs for the development of wound surface model.Methods:A total of 48 sores were prepared on the back of 4 small Bama pigs and divided into 2 groups,namely the Conwell hydrogel group and the Xiangpishengji Ointment group.Photos were taken on the O,7 and 14 days after surgery.Image J software was used to measure the wound area,calculate the wound healing rate,record the wound healing time,and observe the pathological changes with HE staining.The expression of VEGF-Notch signaling pathway related factors in wound tissue was detected by immunohistochemistry.Results:Xiangpishengji Ointment could promote the wound healing of experimental pigs,improve the wound healing rate,shorten the healing time,and promote the expression of VEGF-Notch signaling pathway related factors.Conclusion:Xiangpishengji Ointment can promote the healing of small Bama pig wound model,which may be related to the influence on VEGF-Notch signaling pathway.Experimental pigs are more similar to humans than rats in physiological and pathological aspects,and may become an accurate model for wound healing exploration in the future.

关 键 词：橡皮生肌膏 小型巴马香猪 创面模型制备 VEGF-Notch信号通路 

分 类 号：R965.1[医药卫生—药理学]