辐射诱变筛选高生长速率的渐狭蜡蚧菌及其生物学特性研究  

Screening of Lecanicillium attenuatum with High Growth Rate by Radiation Mutation and Its Biological Characteristics

作　　者：段珂屹 刘安 陈亮[2] 武小芬[2] 齐慧[2] 张祺玲[2] 王振 邓明[2] 王克勤[2] 刘素纯 DUAN Keyi;LIU An;CHEN Liang;WU Xiaofen;QI Hui;ZHANG Qiling;WANG Zhen;DENG Ming;WANG Keqin;LIU Suchun(College of Food Science and Technology,Hunan Agricultural University,Changsha 410125,China;Engineering Technology Research Center of Agricultural Biological Irradiation,Hunan Institute of Nuclear Agricultural Science and Space Mutation Breeding,Hunan Academy of Agricultural Sciences,Changsha 410125,China)

机构地区：[1]湖南农业大学食品科学技术学院,长沙410125 [2]湖南省农业科学院,湖南省核农学与航天育种研究所,长沙410125

出　　处：《激光生物学报》2023年第5期431-440,共10页Acta Laser Biology Sinica

基　　金：国家重点研发计划项目(2021YFE0111200-2);湖南省农业科技创新项目(2022CX119)。

摘　　要：本试验利用电子束及γ射线两种射线对渐狭蜡蚧菌(Lecanicillium attenuatum)3166进行诱变处理,研究适于渐狭蜡蚧菌诱变选育的吸收剂量以及诱变菌株生物学特性。结果表明:电子束及γ射线诱变渐狭蜡蚧菌的最适剂量均为200 Gy,渐狭蜡蚧菌的电子束辐射敏感性D10值为191 Gy、γ射线D10值为366 Gy;以产孢速率为指标筛选获得突变菌株8株,其中5株的菌丝生长及产孢速率较出发菌株显著提高,均为电子束诱变所得,产孢速率分别较出发菌株提高了30.45%、31.55%、23.66%、64.83%、68.77%;诱变菌株的胞外几丁质酶比活在培养5 d时达到峰值,诱变株Ⅱ111、Ⅱ164、Ⅱ181的几丁质酶比活分别较出发菌株显著提高29.29%、54.45%、19.18%,其中Ⅱ164酶比活可达1707.41 U/mg prot;胞外蛋白酶比活在培养7 d时达到峰值,Ⅱ111、Ⅱ164、Ⅱ181的蛋白酶比活分别较出发菌株显著提高17.98%、13.17%、16.50%,其中Ⅱ111酶比活达到30.71 U/mg prot,且渐狭蜡蚧菌诱变株生长速率与胞外酶比活有强相关性。本研究为渐狭蜡蚧菌后续的害虫防治及开发利用提供了理论基础。In this study,electron beam andγ-ray were used to mutagenize Lecanicillium attenuatum 3166,and the absorbed dose suitable for mutation breeding of Lecanicillium attenuatum and the biological characteristics of the mutagenized strain were studied.The results showed that the optimal dose for electron beam andγ-ray mutagenesis of Lecanicillium attenuatum was 200 Gy,the electron beam D10 value of Lecanicillium attenuatum was 191 Gy,and theγ-ray D10 value was 366 Gy;eight mutant strains were screened and obtained with spore production rate as the index.Among them,the hypha growth and spore production rate of five strains significantly increased compared with the parent strain,which were all obtained by electron beam mutagenesis.The spore production rate increased by 30.45%,31.55%,23.66%,64.83%and 68.77%respectively compared with the parent strain.The extracellular chitinase activity of the mutant strain reached the peak after five days of culture,and the chitinase activities of mutant strainsⅡ111,Ⅱ164 andⅡ181 increased by 29.29%,54.45%and 19.18%respectively,compared with that of the parent strain.The chitinase activity of mutant strainⅡ164 reached 1707.41 U/mg prot;the extracellular protease activity reached its peak after 7-day culture,and the protease activities ofⅡ111,Ⅱ164 andⅡ181 were significantly increased by 17.98%,13.17%and 16.50%respectively as compared with those of the parent strain,wherein the enzyme activity ofⅡ111 could reach 30.71 U/mg prot,and the growth rate of the mutant strain had a strong correlation with the extracellular enzyme activity.This study provides a theoretical basis for the follow-up pest control,development and utilization of Lecanicillium attenuatum.

关 键 词：渐狭蜡蚧菌 辐射诱变 突变菌株 生物学特性 胞外酶比活 

分 类 号：TL99[核科学技术—核技术及应用] S476.1[农业科学—农业昆虫与害虫防治]