Streamlined and on-demand preparation of mRNA products on a universal integrated platform  

作　　者：Hongjuan Wei Zhen Rong Liyan Liu Ye Sang Jing Yang Shengqi Wang 

机构地区：[1]Bioinformatics Center of AMMS,Beijing 100850,P.R.China

出　　处：《Microsystems & Nanoengineering》2023年第4期119-128,共10页微系统与纳米工程（英文）

基　　金：This study was supported by the National Key Research and Development Program of China(Grant no.2021YFC2302405).

摘　　要：Vaccines are used to protect human beings from various diseases.mRNA vaccines simplify the development process and reduce the production cost of conventional vaccines,making it possible to respond rapidly to acute and severe diseases,such as coronavirus disease 2019.In this study,a universal integrated platform for the streamlined and ondemand preparation of mRNA products directly from DNA templates was established.Target DNA templates were amplified in vitro by a polymerase chain reaction module and transcribed into mRNA sequences,which were magnetically purified and encapsulated in lipid nanoparticles.As an initial example,enhanced green fluorescent protein(eGFP)was used to test the platform.The expression capacity and efficiency of the products were evaluated by transfecting them into HEK-293T cells.The batch production rate was estimated to be 200–300μg of eGFP mRNA in 8 h.Furthermore,an mRNA vaccine encoding the receptor-binding domain(RBD)of the severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)spike protein was produced by this platform.The proposed integrated platform shows advantages for the universal and on-demand preparation of mRNA products,offering the potential to facilitate broad access to mRNA technology and enable the development of mRNA products,including the rapid supply of new mRNA-based vaccines in pandemic situations and personalized mRNA-based therapies for oncology and chronic infectious diseases,such as viral hepatitis and acquired immune deficiency syndrome.

关 键 词：PREPARATION TEMPLATE offering 

分 类 号：TB383[一般工业技术—材料科学与工程]