Genomic similarity and antibody-dependent enhancement of immune serum potentially affect the protective efficacy of commercial MLV vaccines against NADC30-like PRRSV  

作　　者：Yang Li Lele Xu Dian Jiao Zifang Zheng Zhihao Chen Yang Jing Zhiwei Li Zhiqian Maa Yingtong Feng Xuyang Guo Yumiao Wang Yuan He Haixue Zheng Shuqi Xiao 

机构地区：[1]State Key Laboratory for Animal Disease Control and Prevention,College of Veterinary Medicine,Lanzhou University,Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou,730046,China [2]College of Veterinary Medicine,Northwest A&F University,Yangling,712100,China

出　　处：《Virologica Sinica》2023年第5期813-826,共14页中国病毒学（英文版）

基　　金：This research was supported by the National Natural Science Foundation of China(32172846);the Earmarked Fund for CARS-35,the Science and Technology Major Project of Gansu Province(22ZD6NA001);the Science Foundation for Distinguished Young Scholars of Shaanxi Province(2021JC-18);the Natural Science Foundation of Gansu Province(23JRRA1153);the Science and Technology Plan Project of Gansu Province(23JRRA561);the Chinese Academy of Agricultural Science and Technology Innovation Project(CAAS-ASTIP-JBGS-20210602);the Strategic Priority Research Program of the National Center of Technology Innovation for Pigs(NCTIP-XD/C03).

摘　　要：Porcine reproductive and respiratory syndrome(PRRS)is one of the most significant diseases affecting the pig industry worldwide.The PRRSV mutation rate is the highest among the RNA viruses.To date,NADC30-like PRRSV and highly pathogenic PRRSV(HP-PRRSV)are the dominant epidemic strains in China;however,commercial vaccines do not always provide sufficient cross-protection,and the reasons for insufficient protection are unclear.This study isolated a wild-type NADC30-like PRRSV,SX-YL1806,from Shaanxi Province.Vaccination challenge experiments in piglets showed that commercial modified live virus(MLV)vaccines provided good protection against HP-PRRSV.However,it could not provide sufficient protection against the novel strain SXYL1806.To explore the reasons for this phenomenon,we compared the genomic homology between the MLV strain and HP-PRRSV or NADC30-like PRRSV and found that the MLV strain had a lower genome similarity with NADC30-like PRRSV.Serum neutralization assay showed that MLV-immune serum slightly promoted the homologous HP-PRRSV replication and significantly promoted the heterologous NADC30-like PRRSV strain replication in vitro,suggesting that antibody-dependent enhancement(ADE)might also play a role in decreasing MLV protective efficacy.These findings expand our understanding of the potential factors affecting the protective effect of PRRSV MLV vaccines against the NADC30-like strains.

关 键 词：NADC30-like PRRSV Modified live virus(MLV)vaccines Genomic similarity Antibody dependent enhancement(ADE) Cross protection 

分 类 号：R392-33[医药卫生—免疫学]