从海马区胶质细胞CD137L途径探讨电针治疗神经痛的作用机制  

Mechanism of Electroacupuncture on Neuropathic Pain via Regulation of CD137L in Hippocampal Glia

作　　者：郑昌岳[1] 兰艳艳 黄秋玲 江孟鸿 王志福 ZHENG Changyue;LAN Yanyan;HUANG Qiuling;JIANG Menghong;WANG Zhifu(Fujian Provincial Government Hospital,Fuzhou,Fujian 350003,China;Rehabilitation Hospital Affiliated to Fujian University of Traditional Chinese Medicine,Fuzhou,Fujian 350003,China;College of Acupuncture and Moxibustion,Fujian University of Traditional Chinese Medicine,Fuzhou,Fujian 350122,China)

机构地区：[1]福建省级机关医院,福建福州350003 [2]福建中医药大学附属康复医院,福建福州350003 [3]福建中医药大学针灸学院,福建福州350122

出　　处：《康复学报》2023年第5期419-427,共9页Rehabilitation Medicine

基　　金：国家自然科学基金面上项目(82274658)。

摘　　要：目的:探讨海马CA1区胶质细胞CD137L在电针改善神经痛大鼠机械痛及学习记忆障碍的作用机制。方法:将24只SD大鼠按照随机数字表法分为假手术组、模型组和电针组,每组8只。模型组和电针组制备坐骨神经分支选择性损伤(SNI)神经痛模型;假手术组只暴露坐骨神经,但不结扎。造模后第7天开始,电针组对右侧环跳、阳陵泉穴予以电针刺激,频率2 Hz,强度1 mA,每天1次,每次30 min,连续21 d;假手术组和模型组同等条件抓取、固定,但不进行任何干预。SD大鼠造模前及造模后第28天,用Von-Frey测痛仪检测机械痛阈值;造模后28 d采用新物体识别实验检测大鼠学习记忆能力;酶联免疫吸附法检测海马CA1区CD137L、胶质细胞活化标记物(Iba-1、GFAP)和肿瘤坏死因子-α(TNF-α)的浓度;免疫荧光组织化学法检测海马CA1区CD137L及其受体CD137在神经细胞的表达定位情况。结果:①与假手术组比较,模型组机械痛阈值和新物体识别指数均显著降低(P<0.01);与模型组比较,电针组机械痛阈值和新物体识别指数均显著升高(P<0.01)。②与假手术组比较,模型组海马CD137L、Iba-1、GFAP和TNF-α浓度均显著升高(P<0.01),Iba-1和GFAP免疫反应阳性个数明显增多(P均<0.01);与模型组比较,电针组海马CD137L、Iba-1、GFAP和TNF-α浓度均显著下降(P均<0.05),Iba-1和GFAP免疫反应阳性个数均明显减少(P<0.01)。③免疫荧光检测发现,海马CA1区CD137L及其受体CD137与Iba-1、GFAP共表达明显,提示CD137L及其受体CD137主要表达于小胶质细胞、星形胶质细胞,但不表达于神经元。结论:电针可能下调海马CA1区胶质细胞CD137L表达,抑制胶质细胞的活化和TNF-α的释放,减轻神经痛大鼠疼痛及学习记忆障碍。Objective:To investigate the mechanism of CD137L in CA1 region of hippocampus in improving mechanical pain and memory impairment in neuropathic pain rats during electroacupuncture therapy.Methods:A total of 24 SD rats were randomly divided into sham operation group,model group and electroacupuncture group,with 8 rats in each group.In the model and electroacupuncture groups,the spared nerve injury(SNI)model was made.The sham operation group was just exposed right sciatic nerve without ligation.From the 7th day after modeling,the electroacupuncture group received electroacupuncture intervention in the right side Huantiao(GB30)and Yanglingquan(G34)acupoints,30 minutes each time,once a day,lasting for 21 days.Rats in the model and the sham operation groups were equally grasped and fixed without any intervention.Von-Frey pain detector was used to measure the mechanical withdrawal threshold of rats.On the 28th day after modeling,new object recognition test was used to detect the learning and memory ability.The expression of hippocampal CA1 region CD137L,Iba-1,GFAP and tumor necrosis factor-α(TNF-α)was detected by enzyme-linked immunosorbent assay(ELISA).Immunofluorescence was used for the cellular localization of CD137L and receptor CD137,and to measure the Iba-1 and GFAP expression in hippocampal CA1 region.Results:1)Compared with the sham operation group,mechanical pain threshold and new object recognition index in the model group significantly decreased(P<0.01);compared with the model group,mechanical pain threshold and new object recognition index in the electroacupuncture group significantly increased(P<0.01).2)Compared with the sham operation group,the concentrations of CD137L,Iba-1,GFAP and TNF-αin hippocampus of rats in the model group significantly increased(P<0.01),and the number of positive Iba-1 and GFAP immune reactions significantly increased(P<0.01);compared with the model group,the concentrations of CD137L,Iba-1,GFAP and TNF-αin hippocampus of rats in the electroacupuncture group significantly decre

关 键 词：神经痛 海马 CD137L 胶质细胞 电针 

分 类 号：R245[医药卫生—针灸推拿学]