电针风池穴对颈肌慢性损伤大鼠肌卫星细胞及TLR4/MyD88/NF-κB信号通路的影响  

Effects of Electroacupuncture at Fengchi Acupoint on Myosatellite Cells and TLR4/MyD88/NF-κB Signaling Pathway in Chronic Cervical Muscle Injury Rats

作　　者：黄于婷 陈佳彦 阚宇 方燕平[1] 王金伟 郑林遥 景向红[3] 廖军[1] HUANG Yuting;CHEN Jiayan;KAN Yu;FANG Yanping;WANG Jinwei;ZHENG Linyao;JING Xianghong;LIAO Jun(College of Acupuncture and Moxibustion,Fujian University of Traditional Chinese Medicine,Fuzhou,Fujian 350122,China;Sichuan Health Rehabilitation Vocational College,Zigong,Sichuan 643000,China;Institute of Acupuncture and Moxibustion,China Academy of Chinese Medical Sciences,Beijing 100700,China)

机构地区：[1]福建中医药大学针灸学院,福建福州350122 [2]四川省卫生康复职业学院,四川自贡643000 [3]中国中医科学院针灸研究所,北京100700

出　　处：《康复学报》2023年第5期428-435,共8页Rehabilitation Medicine

基　　金：国家自然科学基金项目(82074181);福建省自然科学基金项目(2023J01151);福建省教育厅中青年教师项目(JAT190233);福建中医药大学校管课题(X2019010-人才专项);福建省康复重点实验室联合福建省康复产业研究院开放课题(2015Y2001)。

摘　　要：目的:观察电针风池穴对颈肌慢性损伤大鼠肌卫星细胞、生肌决定因子(MyoD)、增殖细胞核抗原(PCNA)、Toll样受体-4(TLR4)、髓样分化因子88(MyD88)及核转录因子(NF-κBp65)蛋白表达的影响,探讨电针促进肌肉慢性损伤修复的可能机制。方法:从45只Wistar大鼠中选取10只作为空白组,将剩余大鼠造模后按照随机数字表法分为模型组、电针组和美洛昔康组,每组10只(5只造模不成功)。采用长时间低头固定并注射高渗盐水的方法复制大鼠颈肌慢性损伤模型。造模3个月后,使用超声诊断仪观察大鼠颈后肌的形态学变化;用肌电生理检测验证模型是否成功。电针组电针双侧风池穴,每次25 min,每日1次,连续治疗10 d为1个疗程,疗程之间间隔2 d,共治疗2个疗程。干预结束后,取各组大鼠颈后肌组织,在电镜下观察颈后肌组织的形态学变化;采用免疫组织化学法检测PCNA、MyoD、TLR4、MyD88及NF-κBp65蛋白的含量。结果:①形态学检测:与空白组相比,模型组的超声诊断结果显示大鼠颈后肌组织不连续,厚度明显变薄;肌电生理检测结果表明模型组肌电波幅及频率较空白组明显衰减;与空白组相比,模型组的肌肉电镜结果显示肌原纤维结构的排列不连续,部分线粒体肿胀,肌纤维和肌节之间有断裂,并有空泡现象存在;与模型组相比,电针组肌原纤维清晰可见,肌丝横断面呈点状分布,肌节和肌浆膜完整,个别线粒体肿胀,其中少量与肌原纤维的断裂处有空泡;美洛昔康组肌原纤维结构偶见不规则,在肌原纤维的断裂处有空泡,出现少量的线粒体肿胀。②免疫组织化学:与空白组相比,模型组中的PCNA、MyoD的表达呈现弱阳性,TLR4、MyD88、NF-κBp65的蛋白表达量明显增加(P<0.05);与模型组比较,电针组与美洛昔康组中PCNA和MyoD的蛋白表达量明显增加(P<0.05),TLR4、MyD88、NF-κBp65的蛋白表达量明显减少(P<0.05)。电针组与美洛昔康Objective:To observe effects of electroacupuncture at Fengchi acupoint on expression of myosatellite cells,myogenic determining factor(MyoD),proliferating cell nuclear antigen(PCNA),Toll-like receptor 4(TLR4),myeloid differentiation primary response gene 88(MyD88)and nuclear factor-kappa B p65(NF-κBp65)protein in rat with chronic cervical muscle injury,so as to explore possible mechanism of electroacupuncture in promoting the repair of chronic cervical muscle injury.Methods:Ten Wistar rats were selected from 45 Wistar rats as blank group,and the remaining rats were divided into model group,electroacupuncture group(EA group)and meloxicam group according to random number table method,with 10 rats in each group(5 rats were unsuccessful in modeling).Chronic cervical muscle injury model of rats was established by long-term head flexion fixation and hypertonic saline injection.After 3 months of modeling,the morphological changes of posterior cervical muscle were observed by ultrasonic diagnostic detector.The success of modeling was verified by EMG.The EA group received electroacupuncture at Fengchi acupoint on both sides,25 min each time,once a day,for 10 consecutive days as one course of treatment,with an interval of two days between courses of treatment,for 2 courses of treatment.After intervention,the posterior cervical muscle tissue of rats in each group was collected and the morphological changes of posterior cervical muscle tissue were observed under electron microscope.The protein contents of PCNA,MyoD,TLR4,MyD88 and NF-κBp65 were detected by immunohistochemistry.Results:1)Morphological detection:Compared with the blank group,ultrasonic diagnosis results of the model group showed that tissue of posterior cervical muscle of rats was discontinuous and thickness was significantly narrower.Electrophysiological test results showed that amplitude and frequency of electromyographic significantly decreased in the model group.Compared with the blank group,muscle electron microscopy results of the model group showed tha

关 键 词：颈肌慢性损伤 电针 肌卫星细胞 MYOD TLR4/MyD88/NF-κB信号通路 

分 类 号：R245[医药卫生—针灸推拿学]