Calorie restriction potentiates epigallocatechin-3-gallate-mediated Nrf2 activation in hepatocytes of aged rats  

作　　者：Rajeswari Ravindran Malathi Manuel Thangarajeswari Mohan Ravindran Jaganathan Kalaiselvi Periandavan 

机构地区：[1]Preclinical Department,Faculty of Medicine,Universiti Kuala Lumpur,Royal College of Medicine Perak,30450,Ipoh,Malaysia [2]Department of Medical Biochemistry,Dr A.L Mudaliar Institute of Basic Medical Sciences,University of Madras,Taramani,Chennai 600113,India

出　　处：《Asian Pacific Journal of Tropical Biomedicine》2023年第10期421-430,共10页亚太热带生物医学杂志（英文版）

基　　金：The article was financially supported by UGC SAP Programme,University of Madras,Chennai,India.

摘　　要：Objective:To explore the combinatorial effect of epigallocatechin-3-gallate(EGCG)and calorie restriction on activation of nuclear factor erythroid 2-related factor 2(Nrf2),a transcription factor involved in the antioxidant defense system of aged rats.Methods:Aged male Wistar rats were calorie-restricted and treated with EGCG orally for 45 days.The initial body weight of aged rats was recorded,and the final body weight was measured at the end of the experimental period.Serum lipid and lipoprotein status,oxidative stress markers such as free radicals and malondialdehyde levels,and reduced glutathione were assessed.In addition,RT-PCR and Western blotting analyses were performed.Results:Calorie restriction potentiated the effect of EGCG on enhancing antioxidant status,improving the levels of serum lipid and lipoproteins,upregulating Nrf2 and Bcl2,and downregulating Keap1,cullin3,Bax and cytochrome c in aged rats.Conclusions:Calorie restriction can promote EGCG-mediated Nrf2 activation in aged rats.This preliminary finding paves the way for a combinatory approach to replenishing the antioxidant status during aging,thereby reducing the risk for age-associated degenerative diseases.

关 键 词：Aging Reactive oxygen species Calorie restriction EGCG NRF2 ANTIOXIDANT 

分 类 号：R285.5[医药卫生—中药学]