基于OSCE在真实世界案例探索制定地西泮注射液配制规则  

Exploration and formulation of diazepam injection preparation rules based on OSCE in real world cases

作　　者：康力敏 尹辰 董旺青 沈倩 冯刘欣 余静洁 KANG Limin;YIN Chen;DONG Wangqing;SHEN Qian;FENG Liuxin;YU Jingjie(the Second Affiliated Hospital of Xi'an Jiaotong University,Xi'an 710003;Drug and Vaccine InspectionCenter of Shaanxi Province,Xi'an 710065,China)

机构地区：[1]西安交通大学第二附属医院,陕西西安710003 [2]陕西省药品和疫苗检查中心,陕西西安710065

出　　处：《临床医学研究与实践》2023年第31期182-185,共4页Clinical Research and Practice

基　　金：陕西省自然科学基础研究计划项目(No.2021JQ-425);西安交通大学第二附属医院科研基金自由探索项目[No.2020YJ(ZYTS)030]。

摘　　要：目的探索地西泮注射液最优配制办法,客观规范地制定客观结构化临床考核(OSCE)考核标准,为临床安全用药提供参考。方法通过检索文献、开展实验进行配制证据收集,将地西泮注射液分别用5%葡萄糖注射液(GS)、10%GS及0.9%氯化钠注射液(NS)进行1∶1、1∶5、1∶10、1∶20、1∶25、1∶30、1∶35、1∶40三组稀释对比,肉眼观察微粒的溶解随时间的变化趋势,借助紫外-可见分光光度计(UV-vis)进行吸光度测定,获取最优配制比。结果5%GS组和0.9%NS组在不同稀释倍数下肉眼观测微粒溶解度接近,且在稀释倍数≥25呈现澄清且静置4 h后未见沉淀析出,均明显优于10%GS组。UV-vis显示,相同稀释倍数下5%GS组稀释后紫外可见光吸收强度明显低于0.9%NS组。结论药师OSCE考核需基于临床且提供客观标准化的参考;低稀释倍数三种液体均出现浑浊,静置后出现沉淀,当稀释倍数≥25倍时,三种稀释液未出现明显差异,0.9%NS组在相同稀释倍数下样品的澄明度更高。Objective To explore the optimal preparation method of diazepam injection,and to formulate the objective structured clinical examination(OSCE)assessment standard objectively and normatively,so as to provide reference for clinical safe medication.Methods Prepare evidence was collected by searching literature and conducting experiments.Diazepam injection was diluted with 5% glucose solution(GS),10%GS and 0.9% normal saline(NS)at 1∶1,1∶5,1∶10,1∶20,1∶25,1∶30,1∶35,1∶40,respectively.The change trend of particle dissolution with time was observed by naked eye.The absorbance was measured by ultraviolet-visible spectrophotometer(UV-vis)to obtain the optimal formulation ratio.Results The solubility of 5%GS group and 0.9%NS group was close to that of the naked eye at different dilution times,and it was clear at dilution times≥25 and no precipitation was observed after standing for 4 h,which was significantly better than that of 10%GS group.UV-vis showed that the UV-visible absorption intensity of 5%GS group was significantly lower than that of 0.9%NS group at the same dilution ratio.Conclusion The OSCE assessment of pharmacists should be based on clinical practice and provide objective and standardized reference.The three liquids with low dilution multiples showed turbidity,and sedimentation occurred after standing.When the dilution multiple was≥25 times,there was no significant difference among the three diluents,the clarity of 0.9%NS group was higher at the same dilution multiple.

关 键 词：客观结构化临床考核 地西泮注射液 稀释倍数 澄明度 

分 类 号：R971[医药卫生—药品]