HPLC和UPLC-MS/MS法测不同水体中四环素类抗生素残留的比较研究  

Comparative Study on Determination of Tetracycline Antibiotic Residues in Different Water Bodies by HPLC and UPLC-MS/MS

作　　者：蔡鹏 盛天露 刘华[2] 陈冠宜 易永 刘永林 王丽阳 陈刚 Cai Peng;Sheng Tian-lu;Liu Hua;Chen Guan-yi;Yi Yong;Liu Yong-lin;Wang Li-yang;Chen Gang(Development Research institute of Testing and Certification Technology,Jiangxi General institute of Testing And Certification,Nanchang Jiangxi 330052,China;College of Pharmacy,Jiangxi University of Traditional Chinese Medicine,Nanchang 330004,China;Nanchang Medical College,Nanchang Jiangxi 330004,China)

机构地区：[1]江西省检验检测认证总院检测认证技术发展研究院,江西南昌330052 [2]江西中医药大学药学院,江西南昌330004 [3]南昌医学院,江西南昌330004

出　　处：《江西化工》2023年第5期22-25,共4页Jiangxi Chemical Industry

基　　金：国家自然科学基金地区项目(81760706);2021年度江西省中医药科技计划一般项目(2021A347)。

摘　　要：目的:建立高效液相色谱法(HPLC)定量测定不同水体中四环素类抗生素的残留量;方法:同时采用超高液相色谱-串联质谱法(UPLC-MS/MS)进行定性确认及定量检测,并从方法线性范围、检出限、精密度,加标回收率等角度出发,比较两者的异同点;结果:无论采用HPLC还是UPLC-MS/MS检测,两者四环素类目标物的线性关系均良好(r>0.999),在加标回收率实验中,两种方法的回收率均在85.0%~100.9%,相对标准偏差均在0.46%~4.15%,目标化合物在HPLC的检出限均低于0.1mg/L,在UPLC-MS/MS中的检出限均低于0.8μg/L;结论:两种方法皆符合相关的检测方法要求,适合针对不同浓度目标物的水体进行测定。High performance liquid chromatography(HPLC)was established for quantitative determination of tetracycline antibiotic residues in different water bodies,and ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)was used for qualitative confirmation and quantitative detection.The similarities and differences between the two methods were compared from the aspects of linear range,detection limit,precision and recovery rate.Results both HPLC and UPLC-MS/MS showed a good linear relationship(r>0.999).in the recovery experiment,the recoveries of the two methods were between 85.0%and 100.9%,the relative standard deviations were between 0.46%and 4.15%,and the detection limits of the target compounds in HPLC were lower than 0.1 mg/L.Conclusion Both methods meet the requirements of relevant detection methods and are suitable for the determination of water with different concentrations of target substances.

关 键 词：HPLC UPLC-MS/MS 四环素 比较研究 

分 类 号：X832[环境科学与工程—环境工程]