HIF-1α、HIF-2α及OPA1基因在藏绵羊肺脏中的表达特征分析  

The Expression Characteristics of HIF-1α,HIF-2αand OPA1 Genes in Lung of Tibetan Sheep

作　　者：席启明 赵芳芳 何兆华 孙红先李 少斌 XI Qiming;ZHAO Fangfang;HE Zhaohua;SUN Hongxian;LI Shaobin(Gansu Police Vocational College,Lanzhou 730070,China;College of Animal Science and Technology,Gansu Key Laboratory of Herbivorous Animal Biotechnology,Gansu Agricultural University,Lanzhou 730070,China)

机构地区：[1]甘肃警察职业学院,甘肃兰州730070 [2]甘肃农业大学动物科学技术学院/甘肃省草食动物生物技术重点实验室,甘肃兰州730070

出　　处：《高原科学研究》2023年第3期32-38,共7页Plateau Science Research

基　　金：甘肃省教育厅创新能力提升项目(2019A-050);甘肃农业大学伏羲人才项目(GAUFX-03Y04)。

摘　　要：试验通过RT-qPCR和ELISA方法分别测定了HIF-1α、HIF-2α及OPA1在不同海拔梯度(2500 m、3500 m、4500 m)和年龄段(4月龄、1.5岁、3.5岁)藏绵羊肺脏中的mRNA和蛋白质表达水平,分析HIF-1α、HIF-2α及OPA1表达特征在藏绵羊高原低氧适应中的作用模式。结果显示:在不同海拔梯度和年龄段,HIF-1α、HIF-2α及OPA1基因mRNA与蛋白质表达趋势基本一致;随着海拔的升高,HIF-1α和HIF-2α基因与蛋白表达量呈递增趋势,OPA1基因与蛋白表达量呈递减趋势,且HIF-1α和HIF-2α基因表达量随着海拔的升高显著增加(P<0.05),OPA1基因的表达量随着海拔的升高显著减少(P<0.05),OPA1蛋白在2500 m海拔的表达量显著高于4500 m海拔的表达量(P<0.05);随着年龄的增加,HIF-1α和HIF-2α基因与蛋白表达量呈递增趋势,OPA1基因与蛋白表达量呈递减趋势,且HIF-1α基因表达量随着年龄的增加而显著增加(P<0.05),OPA1基因表达量随着年龄的增加而显著减少(P<0.05)。同时,在不同海拔梯度和年龄段,HIF-1α蛋白表达量都明显低于HIF-2α和OPA1蛋白的表达量。以上结果提示,HIF-1α、HIF-2α及OPA1基因和蛋白质表达水平的海拔差异性和年龄差异性有可能是低氧环境下维持生物体功能活动正常运转的分子基础。In this study,the mRNA and protein expression levels of HIF-1α,HIF-2αand OPA1 in lung of Tibetan sheep at different altitudes(2500 m,3500 m,4500 m)and ages(4 months,1.5 years,3.5 years)were determined by real-time quantitative PCR(RT-QPCR)and enzyme-linked immunosorbent assay(ELISA),and their roles and significance in adaptive to altitude hypoxia mechanism were analyzed according to their expression differences.The results showed that the expression trends of HIF-1α,HIF-2αand OPA1 genes and proteins were basically consistent at different altitudes and ages.The expression levels of HIF-1αand HIF-2αgenes and proteins were significantly increased(P<0.05),while the expression levels of OPA1 gene and protein were significantly decreased(P<0.05)with the increase of altitude..The expression of OPA1 protein at 2500 m altitude was significantly higher than that at 4500 m altitude(P<0.05).The expression levels of HIF-1αand HIF-2αgenes and proteins were significantly increased(P<0.05)while the expression levels of OPA1 genes and proteins weresignificantly decreased(P<0.05)with the increase of age,and the expression level of HIF-1αproteins was significantly lower than of HIF-2αand OPA1 at different altitudes and ages.These results suggest that the altitude and age differences in the gene and protein expression levels of of HIF-1α,HIF-2αand OPA1 may be the molecular basis related to the maintaining normal functional activities of organisms in hypoxic environments.

关 键 词：藏绵羊 HIF-1Α HIF-2α OPA1 低氧适应 

分 类 号：S826.2[农业科学—畜牧学]