青海部分地区牦牛腐蹄病病原分离及鉴定  

Pathogen isolation and identification of yak footrot in some areas of Qinghai

作　　者：马凯茹 刘双喜 潘复臻 赵猛 赵静[1] 张红见[1] MA Kairu;LIU Shuangxi;PAN Fuzhen;ZHAO Meng;ZHAO Jing;ZHANG Hongjian(College of Agriculture and Animal Husbandry,Qinghai University,Xining 810016,China)

机构地区：[1]青海大学农牧学院,青海西宁810016

出　　处：《青海大学学报》2023年第5期41-45,共5页Journal of Qinghai University

基　　金：青海省科学技术厅项目(2020-HZ-801,2021-HZ-803);青海省农业农村厅项目(NMSY-2020-02,NMSY-2020-03)。

摘　　要：为了解青海部分地区牦牛腐蹄病病原流行情况,本研究对从格尔木市和河南县某牦牛养殖合作社采集的38份牦牛腐蹄病病料进行了常规细菌分离、API20A生化鉴定及16S rDNA基因的PCR扩增与测序。细菌分离结果显示:从格尔木市采集的18份病料中分离出1株坏死梭杆菌,从河南县采集的20份病料中分离出1株魏氏梭菌。测序和序列分析显示:分离获得的2种病原菌的16S rDNA基因序列与GenBank中坏死梭杆菌、魏氏梭菌序列间的同源性分别为33%~35%、98%~100%。研究结果可为后期牦牛腐蹄病的预防和控制提供理论参考。In order to investigate the epidemic situation of yak footrot pathogen in some areas of Qinghai,38 samples collected from yak breeding cooperatives in Golmud and Henan county are subjected to routine bacterial isolation,API20A biochemical identification,and 16S rDNA gene PCR amplification and sequencing.The results of bacterial isolation show that one Fusobacterium necrophorum strain is isolated from 18 samples collected in Golmud,and one Clostridium perfringens strain is isolated from 20 samples collected in Henan county.Sequence analysis show that the 16S rDNA gene sequences of the two isolated pathogens have 33%~35%and 98%~100%homology with the sequences of Fusobacterium necrophorum and Clostridium perfringens in GenBank respectively.The results can provide a certain theoretical reference for the prevention and control of yak footrot in the future.

关 键 词：腐蹄病 病原菌 16S rDNA PCR 分离鉴定 

分 类 号：S855.1[农业科学—临床兽医学] S852.6[农业科学—兽医学]