基于Hela细胞的失巢凋亡现象时序评价模型的建立及测量验证方法研究  

Establishment of Measurement and Verification Method forSequential Evaluation of Anoikis Effect on Hela Cell

作　　者：薛志超[1] 曾嘉明 龚晓云[1] 薛倩 戴新华[1] 赵洋 XUE Zhi-chao;ZENG Jia-ming;GONG Xiao-yun;XUE Qian;DAI Xin-hua;ZHAO Yang(Technology Innovation Center of Mass Spectrometry for State Market Regulation,Center for Advanced Measurement Science,National Institute of Metrology,Beijing 100029,China)

机构地区：[1]中国计量科学研究院前沿计量科学中心国家市场监管技术创新中心(质谱),北京100029

出　　处：《计量学报》2023年第10期1612-1616,共5页Acta Metrologica Sinica

基　　金：中国计量科学研究院院基本科研业务费(AKYZZ2224)。

摘　　要：失巢凋亡作为一种特殊的程序化细胞死亡形式,在机体发育、组织自身平衡、疾病发生和肿瘤转移中起重要作用。利用贴壁增殖表皮细胞系海拉(Hela)细胞作为模型,通过对比贴壁细胞悬浮后悬浮时间对细胞活性的影响,深入探讨了细胞失巢凋亡现象。研究考察了悬浮5 min、30 min、1 h, 2 h, 4 h和8 h后的活细胞数量,以及重新贴壁培养后对细胞活性(即贴壁24 h后活细胞增殖数量)的影响。结果表明:悬浮时间可导致海拉细胞数量减少及活性降低,在细胞实验过程中应当严格控制悬浮细胞接种时长。Anoikis effect is a special program death for attachment cells in the body,which plays an important role in body development,tissue homeostasis,disease occurrence,and tumor metastasis.Attachment epithelial cell line Hela was hired as the experiment model to investigate the anoikis effect in detail.Living Hela cells were counted after trypsinization and floating for 5 min,30 min,1 h,2 h,4 h,and 8 h respectively.The activity of these cells was detected after seeding back for 24 h.We found that floating time would severely decrease cell counting as well as cell activity.The floating time of attachment cell needs to be controlled during cell experiments.The results showed that floating time would severely decrease cell counting and activity of HeLa,and the floating time of attachment cell needs to be controlled during cell experiments.

关 键 词：计量学 生命科学计量 细胞数量 细胞活性 细胞测量 失巢凋亡 HELA细胞 悬浮时间 

分 类 号：TB99[一般工业技术—计量学]