蒲公英甾醇对2,4-二硝基氯苯诱导的特应性皮炎小鼠模型的改善作用  

Ameliorative effect of taraxasterol on 2,4-dinitrochlorobenzene induced atopic dermatitis in mice

作　　者：马天怡 毛敏 吴秋月 赵益 朴英实[1,3] 金桂花 任香善(指导)[1,3] MA Tianyi;MAO Min;WU Qiuyue;ZHAO Yi;PIAO Yingshi;JIN Guihua;REN Xiangshan(Cancer Research Center,Department of Pathology,Medical College,Yanbian University,Yanji 133002,China)

机构地区：[1]延边大学医学院病理学教研室肿瘤研究中心,延吉133002 [2]延边大学医学院,延吉133002 [3]延边大学国家民委重点实验室/吉林省科技厅重点实验室,延吉133002 [4]延边大学医学院免疫学与病原生物学教研室,延吉133002

出　　处：《中国免疫学杂志》2023年第10期2160-2165,共6页Chinese Journal of Immunology

基　　金：国家自然科学基金项目(81960305);吉林省大学生创新训练项目(202110184121)。

摘　　要：目的:初步探讨蒲公英甾醇对特应性皮炎小鼠模型的治疗作用及可能的分子机制。方法:选用BALB/c雄性小鼠,采用2,4-二硝基氯苯构建特应性皮炎小鼠模型,并灌胃低浓度和高浓度的蒲公英甾醇进行治疗,观察皮损情况、搔抓次数,测定脏器指数,进行病理学HE染色、CD4^(+)免疫组化实验,ELISA法检测炎症因子TNF-α、IL-4、IL-6水平。结果:蒲公英甾醇能够显著降低小鼠的皮损程度、皮肤厚度、搔抓次数、脏器指数,减少炎症细胞浸润、表皮角化、棘层增厚,降低血清中TNF-α、IL-4、IL-6水平。结论:蒲公英甾醇能够改善小鼠特应性皮炎状况,其机制可能与抑制TNF-α、IL-4、IL-6有关。Objective:To preliminarily explore therapeutic effects and possible molecular mechanisms of taraxasterol on mice model of atopic dermatitis.Methods:BALB/c male mice were selected to establish mice model of atopic dermatitis using 2,4-dinitrochlorobenzene,and were intragastrically administrated with low and high concentrations of taraxasterol.Then the skin lesions,scratching times,organ index were observed,and pathological HE staining and CD4^(+)immunohistochemistry were performed,ELISA was used to detect levels of inflammatory factors TNF-α,IL-4 and IL-6.Results:Taraxasterol could significantly reduce the degree of skin lesions,skin thickness,scratching times and organ index,reduce infiltration of inflammatory cells,keratinization of epidermis,thickening of spinous layer,and reduce levels of TNF-α,IL-4 and IL-6 in serum.Conclusion:Taraxasterol can improve condition of atopic dermatitis in mice,and its mechanism may be related to the inhibition of TNF-α,IL-4 and IL-6.

关 键 词：蒲公英甾醇 特应性皮炎 2 4-二硝基氯苯 炎症因子 

分 类 号：R392.8[医药卫生—免疫学]