黑顺片-大黄对药顺势给药和逆势给药对健康大鼠生理指标的影响  

Effect of homeopathic and countercurrent administration of Heishun Tablets-Radix rhei et rhizome couplet medicines on physical indices in healthy rats

作　　者：甘永金[1] 李羡筠[1] 梁丹玉[1] 黄艳妮 凌健安[1] 蓝保强 盘叶花 潘东 农云开 蒋盛军 GAN Yongjin;LI Xianyun;LIANG Danyu;HUANG Yanni;LING Jian′an;LAN Baoqiang;PAN Yehua;PAN Dong;NONG Yunkai;JIANG Shengjun(Institute of Occupational Health and Toxicity Evaluation Guangxi Zhuang Autonomous Region Institute foRthe Prevention and Treatment of Occupational Disease,Nanning 530021,Guangxi,China;Preparation Center,Guangxi Institute of Chinese Medicine&Pharmaceutical Science,Nanning 530022,Guangxi,China)

机构地区：[1]广西壮族自治区职业病防治研究院职业卫生与毒性评价所,广西南宁市530021 [2]广西壮族自治区中医药研究院制剂中心,广西南宁市530022

出　　处：《广西医学》2023年第16期1977-1981,1991,共6页Guangxi Medical Journal

基　　金：广西壮族自治区中医药研究院自主研究项目(桂中自研2021004)。

摘　　要：目的依据中医药的阴阳药性,探讨黑顺片-大黄对药顺势给药和逆势给药对健康大鼠生理指标的影响。方法选取无特定病原体级SD大鼠48只,随机分为顺势饲喂组、逆势饲喂组、混合饲喂组、对照组,每组12只,雌雄各半。对顺势饲喂组早上给予大黄中药配方颗粒溶液灌胃,下午给予黑顺片中药配方颗粒溶液灌胃;逆势饲喂组给药顺序与顺势饲喂组相反;给予混合饲喂组黑顺片中药配方颗粒和大黄中药配方颗粒混合溶液灌胃,分早晚两次给药;给予对照组早晚灌胃等量超纯水。4组均每周给药5 d,共给药6周。分别于饲喂第1天、第10天、第20天、第30天、第45天(给药6周后再观察3 d)时观察各组大鼠的皮肤毛发、行为状态、兴奋程度、激怒反应、睡眠状态、大便状态。分别于开始饲喂后第2周、第4周、第6周的周一、周三、周五测定大鼠的采食量和体重,饲喂第45天检测大鼠血液常规和血生化指标,计算脏器系数。结果实验期间,各组大鼠的皮肤毛发、行为状态、兴奋程度、激怒反应、睡眠状态、大便状态均未见明显改变。4组大鼠在各个阶段的日均采食量差异均无统计学意义,饲喂第2周、第4周,4组大鼠体重差异无统计学意义(P>0.05)。饲喂第6周,顺势饲喂组、混合饲喂组雌性大鼠的体重低于逆势饲喂组,而混合饲喂组、逆势饲喂组和顺势饲喂组雄性大鼠体重高于对照组(P<0.05)。饲喂第45天,4组雌性、雄性大鼠心、肝、肺、肾、胃脏器系数,白细胞计数、中性粒细胞计数、淋巴细胞计数、红细胞计数,以及血红蛋白、碱性磷酸酶、三酰甘油、尿素、空腹血糖、白蛋白、AST水平差异无统计学意义(P>0.05);逆势饲喂组雌性大鼠脾脏脏器系数高于对照组,顺势饲喂组和混合饲喂组雌性大鼠的单核细胞计数和嗜酸性粒细胞计数高于对照组,顺势饲喂组和混合饲喂组雄性大鼠的嗜酸性�Objective To investigate the effect of homeopathie and countercurrent administration of Heishun Tablets-Radix rhei et rhizome couplet medicines on physical indices in healthy rats according to yin-yang drug properties of Traditional Chinese Medicine.Methods A total of 48 SD rats with specific pathogen free were randomly assigned to homeopathic feeding group,countercurrent feeding group,mixed feeding group,or control group,with 12 rats in each group and half male and hale female.The homeopathic feeding group received intragastric administration of Dahuang Chinese Medicine Formula Granule Solution in the morning,and Heishun Tablet Chinese Medicine Formula Granule Solution in the aftermnoon.The countercurrent feeding group received inverse administration sequence compared with he homeopathic feeding group.The mixed feeding group received intragastric administration of mixed solution of Heishun Tablet Chinese Medicine Formula Granule and Dahuang Chinese Medicine Formula Granule,and the administration was twice in the morning and evening.The control group received intragastric administration of ultrapure water with equivalent volume in the morning and evening.The 4 groups received administration for 5 days every week,and 6 weeks of administration in total.The skin and hair,behavioral state,degree of excitement,irritation reaction,sleep state,and stool state were observed on the first,10^(th),20^(th),30^(th),and 45^(th) day of feeding(observation for three more days after 6 weeks of drug administration),respectively.The feed intake and body weight of rats were measured on Monday,Wednesday and Friday of the second,4^(th) and 6^(th) week after feeding,and on the 45^(th) day of feeding,the blood routine and blood biochemical indices of rats were detected,and the organ coefficient was calculated.Results During experiment,there were no significant changes in the skin and hair,behavioral state,excitement degree,irritation reaction,sleep state,and stool state of rats in various groups.There was no statistically significant di

关 键 词：中医阴阳理论 黑顺片 大黄 分时给药 顺势给药 逆势给药 气滞血瘀 大鼠 

分 类 号：R33[医药卫生—人体生理学] R22[医药卫生—基础医学]