Volatiles of Shiraia fruiting body-associated Pseudomonas putida No.24 stimulate fungal hypocrellin production  

作　　者：Rui Xu Qun Yan Huang Wen Hao Shen Xin Ping Li Li Ping Zheng Jian Wen Wang 

机构地区：[1]College of Pharmaceutical Sciences,Soochow University,Suzhou,215123,China [2]Department of Horticultural Sciences,Soochow University,Suzhou,215123,China

出　　处：《Synthetic and Systems Biotechnology》2023年第3期427-436,共10页合成和系统生物技术（英文）

基　　金：the National Natural Science Foundation of China(No.82073955 and 81773696);the Priority Academic Program Development of the Jiangsu Higher Education Institutes(PAPD).

摘　　要：Hypocrellins are major bioactive perylenequinones from Shiraia fruiting bodies and have been developed as efficient photosensitizers for photodynamic therapy.Pseudomonas is the second dominant genus inside Shiraia fruiting bodies,but with less known actions on the host fungus.In this work,the effects of bacterial volatiles from the Shiraia-associated Pseudomonas on fungal hypocrellin production were investigated.Pseudomonas putida No.24 was the most active to promote significantly accumulation of Shiraia perylenequinones including hypocrellin A(HA),HC,elsinochrome A(EA)and EC.Headspace analysis of the emitted volatiles revealed dimethyl disulfide as one of active compounds to promote fungal hypocrellin production.The bacterial volatiles induced an apoptosis in Shiraia hyphal cell,which was associated with the generation of reactive oxygen species(ROS).ROS generation was proved to mediate the volatile-induced membrane permeability and up-regulation of gene expressions for hypocrellin biosynthesis.In the submerged volatile co-culture,the bacterial volatiles stimulated not only HA content in mycelia,but also HA secretion into the medium,leading to the enhanced HA production to 249.85 mg/L,about 2.07-fold over the control.This is the first report on the regulation of Pseudomonas volatiles on fungal perylenequinone production.These findings could be helpful to understand the roles of bacterial volatiles in fruiting bodies and also provide new elicitation method using bacterial volatiles to stimulate fungal secondary metabolite production.

关 键 词：Shiraia Pseudomonas putida VOLATILES Reactive oxygen species Hypocrellins ELICITATION 

分 类 号：Q68[生物学—生物物理学]