Hematoma-like dynamic hydrogelation through natural glycopeptide molecular recognition for infected bone fracture repair  

作　　者：Shenghao Wang Wenbo He Huan Wang Dachuan Liu Miao Wang Huilin Yang Guoqing Pan Bin Li 

机构地区：[1]Orthopedic Institute,Department of Orthopaedic Surgery,Medical 3D Printing Center,The First Affiliated Hospital,School of Biology&Basic Medical Sciences,SuzhouMedical College,Soochow University,Suzhou,Jiangsu,215006,China [2]Collaborative Innovation Center of Hematology,Soochow University,Suzhou,Jiangsu,215006,China [3]Institute for Advanced Materials,School of Materials Science and Engineering,Jiangsu University,Zhenjiang,Jiangsu,212013,China

出　　处：《Bioactive Materials》2023年第12期73-84,共12页生物活性材料（英文）

基　　金：the National Natural Science Foundation of China(32222041,82102619,81925027,21875092);the Natural Science Foundation of Jiangsu Province(BK20220059);the National Key Research and Development Program of China(2019YFA0112000)。

摘　　要：Infected bone fractures remain a major clinical challenge for orthopedic surgeons.From a tissue regeneration perspective,biomaterial scaffolds with antibacterial and osteoinductive activities are highly desired,while advanced materials capable of mimicking the pathological microenvironment during the healing process of infected tissues remain an area deserving more research.Hematoma,the gel-like blood coagulum,plays an essential role in bone fracture repair because of its ability to serve as a dynamic and temporary scaffold with cytokines for both pathogen elimination and tissue healing.In light of this,we designed a dynamic hydrogel with hematoma-like antimicrobial or reparative performance for infected bone fracture repair in this study.The proposed dynamic hydrogel network was based on the reversible recognition of a natural glycopeptide antibiotic vancomycin(Van)and its target dipeptide D-Ala-D-Ala(AA),which could serve as a hematoma-like scaffold for obliterating bacteria in the fracture region and promoting bone repair by introducing an endogenous osteogenic peptide(OGP).In vivo experiments demonstrated that the hydrogel could rapidly eradicate bacteria,improve bone regeneration and restore the local inflammatory microenvironment.Together,findings from this study imply that the use of hematoma-like dynamic hydrogel could lead to a biomimetic revolution in surgical strategies against susceptible bone fractures.

关 键 词：Dynamic biomaterial Molecular recognition Hematoma mimicking Infected fracture repair Inflammatory microenvironment restoration 

分 类 号：R318[医药卫生—生物医学工程]