Micro-nanofiber composite biomimetic conduits promote long-gap peripheral nerve regeneration in canine models  

作　　者：Xianhao Dong Yueyue Yang Zheheng Bao Adam C.Midgley Feiyi Li Shuxin Dai Zhuangzhuang Yang Jin Wang Lihua Liu Wenlei Li Yayuan Zheng Siyang Liu Yang Liu Weijian Yu Jun Liu Meng Fan Meifeng Zhu Zhongyang Shen Gu Xiaosong Deling Kong 

机构地区：[1]State Key Laboratory of Medicinal Chemical Biology,The Key Laboratory of Bioactive Materials,Ministry of Education,College of Life Science,Nankai University,Tianjin,China [2]Department of Orthopaedics,Tianjin First Central Hospital,School of Medicine,Nankai University,Tianjin,China [3]Department of Radiology,Tianjin First Central Hospital,Tianjin Medical Imaging Institute,School of Medicine,Nankai University,Tianjin,China [4]Institute of Transplantation Medicine,NHC Key Laboratory for Critical Care Medicine,Tianjin First Central Hospital,School of Medicine,Nankai University,Tianjin,China [5]Haihe Laboratory of Sustainable Chemical Transformations,Keyan West Road,Tianjin,300192,China [6]Jiangsu Key Laboratory of Neuroregeneration,Jiangsu Clinical Medicine Center of Tissue Engineering and Nerve Injury Repair,Co-innovation Center ofNeuroregeneration,NMPA Key Laboratory for Research and Evaluation of Tissue Engineering Technology Products,Nantong University,Nantong,China [7]Outpatient Department,Tianjin First Central Hospital,School of Medicine,Nankai University,Tianjin,China [8]Clinical School/College of Orthopedics,Tianjin Medical University,Tianjin,China [9]Department of Joint,Tianjin Hospital,Tianjin,China

出　　处：《Bioactive Materials》2023年第12期98-115,共18页生物活性材料（英文）

基　　金：National Natural Science Foundation of China projects(81921004,D.K.);National Natural Science Foundation of China projects(32201122,X.D.);National Natural Science Foundation of China projects(82272156,M.Z.);China Postdoctoral Science Foundation(2022M711705 X.D.);Key Military Medical Project(No.BLB21J008,D.K.);Tianjin Natural Science Foundation(C100303 F M.).

摘　　要：Peripheral nerve injuries may result in severe long-gap interruptions that are challenging to repair.Autografting is the gold standard surgical approach for repairing long-gap nerve injuries but can result in prominent donor-site complications.Instead,imitating the native neural microarchitecture using synthetic conduits is expected to offer an alternative strategy for improving nerve regeneration.Here,we designed nerve conduits composed of high-resolution anisotropic microfiber grid-cordes with randomly organized nanofiber sheaths to interrogate the positive effects of these biomimetic structures on peripheral nerve regeneration.Anisotropic microfiber-grids demonstrated the capacity to directionally guide Schwann cells and neurites.Nanofiber sheaths conveyed adequate elasticity and permeability,whilst exhibiting a barrier function against the infiltration of fibroblasts.We then used the composite nerve conduits bridge 30-mm long sciatic nerve defects in canine models.At 12 months post-implant,the morphometric and histological recovery,gait recovery,electrophysiological function,and degree of muscle atrophy were assessed.The newly regenerated nerve tissue that formed within the composite nerve conduits showed restored neurological functions that were superior compared to sheaths-only scaffolds and Neurolac nerve conduit controls.Our findings demonstrate the feasibility of using synthetic biophysical cues to effectively bridge long-gap peripheral nerve injuries and indicates the promising clinical application prospects of biomimetic composite nerve conduits.

关 键 词：composite fiber INJURIES 

分 类 号：TB33[一般工业技术—材料科学与工程]