Perfusability and immunogenicity of implantable pre-vascularized tissues recapitulating features of native capillary network  

作　　者：Dhavan Sharma Archita Sharma Linghao Hu Te-An Chen Sarah Voon Kayla J.Bayless Jeremy Goldman Alex J.Walsh Feng Zhao 

机构地区：[1]Department of Biomedical Engineering,Texas A&M University,College Station,TX,United States [2]School of Medicine,Texas A&M University,College Station,TX,United States [3]Department of Biomedical Engineering,Michigan Technological University,Houghton,MI,United States

出　　处：《Bioactive Materials》2023年第12期184-199,共16页生物活性材料（英文）

基　　金：Texas A&M University Microscopy and Imaging Center Core Facility(RRID:SCR_022128);the Integrated Microscopy and Imaging Laboratory at Texas A&M College of Medicine(RRID:SCR_021637)for providing microscopy resources.

摘　　要：Vascularization is a key pre-requisite to engineered anatomical scale three dimensional(3-D)constructs to ensure their nutrient and oxygen supply upon implantation.Presently,engineered pre-vascularized 3-D tissues are limited to only micro-scale hydrogels,which meet neither the anatomical scale needs nor the complexity of natural extracellular matrix(ECM)environments.Anatomical scale perfusable constructs are critically needed for translational applications.To overcome this challenge,we previously developed pre-vascularized ECM sheets with long and oriented dense microvascular networks.The present study further evaluated the patency,perfusability and innate immune response toward these pre-vascularized constructs.Macrophage-co-cultured prevascularized constructs were evaluated in vitro to confirm micro-vessel patency and perturbations in macrophage metabolism.Subcutaneously implanted pre-vascularized constructs remained viable and formed a functional anastomosis with host vasculature within 3 days of implantation.This completely biological pre-vascularized construct holds great potential as a building block to engineer perfusable anatomical scale tissues.

关 键 词：Pre-vascularization Micro-vasculature Mesenchymal stem cells Extracellular matrix Fluorescence lifetime imaging 

分 类 号：R318[医药卫生—生物医学工程]