基于液质联用的金钱草及其习用品的化学成分差异性分析  

Analysis on the difference of chemical constituents in Lysimachiae Herba and its relative byproducts based on LC-MS

作　　者：周永逸 陈海杰 薛佳 袁嘉欢 吴楠 刘训红[1,2] 邹立思 程建明[1,2] ZHOU Yong-yi;CHEN Hai-jie;XUE Jia;YUAN Jia-huan;WU Nan;LIU Xun-hong;ZOU Li-si;CHENG Jian-ming(School of Pharmacy,Nanjing University of Chinese Medicine;Jiangsu Province Engineering Research Center of Classical Prescription,Nanjing 210023,China)

机构地区：[1]南京中医药大学药学院 [2]江苏省经典名方工程研究中心,南京210023

出　　处：《天然产物研究与开发》2023年第10期1669-1678,共10页Natural Product Research and Development

基　　金：江苏高校品牌专业建设工程资助项目(PPZY2015A070)。

摘　　要：基于液质联用(LC-MS)技术,对金钱草及其习用品(小金钱草、连钱草)中化学成分差异性进行研究,为金钱草及其习用品区分鉴别和合理使用提供参考。首先采用超快速液相色谱-三重四级杆飞行时间质谱(UFLC-Triple TOF-MS/MS)结合多元统计分析对金钱草及其习用品中化学成分差异性进行分析,然后建立超快速液相色谱-三重四极杆/线性离子阱质谱(UFLC-QTRAP-MS/MS)同时测定共有差异化学成分含量的方法。结果共鉴定出13个差异化学成分,其中绿原酸、金丝桃苷、芦丁、异槲皮苷、槲皮苷和山柰酚-3-O-芸香糖苷为3者共有差异化学成分;同时测定了这6个共同差异成分的含量,6个成分在相应的浓度范围内线性关系相关系数(r)均大于0.9994,精密度、重复性和稳定性良好,平均回收率为99.77%~101.89%,且相对标准偏差(RSD)为1.27%~2.19%。金钱草中芦丁和山柰酚-3-O-芸香糖苷的含量较高,其余4个成分在小金钱草中含量较高,而连钱草中6个成分的含量均较少。本研究所建立的方法准确、可靠,可为建立金钱草药材的综合品质评价体系提供基础资料。To research the difference of chemical constituents in Lysimachiae Herba and its relative byproducts(Dichondrae Herba and Glechomae Herba)based on LC-MS,and provide reference for the rational use of Lysimachiae Herba and its relative byproducts.The difference of chemical constituents in Lysimachiae Herba and its relative byproducts was analyzed by using ultra-fast liquid chromatography coupled with triple quadrupole-time of flight tandem mass spectrometry(UFLC-Triple TOF-MS/MS)coupled with multivariate statistical analysis.Besides,a method based on ultra-fast liquid chromatography-triple quadrupole-linear ion trap mass spectrometry(UFLC-QTRAP-MS/MS)was developed for the simultaneous determination of common differential constituents in Lysimachiae Herba and its relative byproducts.Finally,a total of 13 constituents were identified and inferred,and six common differential constituents were screened out,which were chlorogenic acid,hyperin,rutin,isoquercitrin,quercetin,and kaempferol 3-O-rutinoside.In order to preliminarily explore the possible reasons for the differences in the efficacy of Lysimachiae Herba and its relative byproducts,the content of six common differential constituents was determined simultaneously.The results showed that the linear correlation coefficients(r)of six constituents were greater than 0.9994 and the method featured a good precision,repeatability,and stability;The average recoveries were between 99.77%and 101.89%,with RSD less than 2.19%.The results showed that the content of rutin and kaempferol 3-O-rutinoside was higher in Lysimachiae Herba,and the other four constituents was higher in Dichondrae Herba,while the content of all six components was lower in Glechomae Herba.The method established in this study is accurate and reliable,and can provide basic information for the establishment of a comprehensive quality evaluation system for Lysimachiae Herba.

关 键 词：金钱草 习用品 液质联用 差异化学成分 含量分析 

分 类 号：R932[医药卫生—生药学]