Mass spectrometry imaging and single-cell transcriptional profiling reveal the tissue-specific regulation of bioactive ingredient biosynthesis in Taxus leaves  

作　　者：Xiaori Zhan Tian Qiu Hongshan Zhang Kailin Hou Xueshuang Liang Cheng Chen Zhijing Wang Qicong Wu Xiaojia Wang Xiao-lin Li Mingshuang Wang Shangguo Feng Houqing Zeng Chunna Yu Huizhong Wang Chenjia Shen 

机构地区：[1]College of Life and Environmental Sciences,Hangzhou Normal University,Hangzhou 311121,China [2]Zhejiang Provincial Key Laboratory for Genetic Improvement and Quality Control of Medicinal Plants,Hangzhou Normal University,Hangzhou 311121,China [3]Kharkiv Institute,Hangzhou Normal University,Hangzhou 311121,China [4]College of Pharmacy,Hangzhou Normal University,Hangzhou 311121,China [5]State Key Laboratory Breeding Base of Dao-di Herbs,National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China

出　　处：《Plant Communications》2023年第5期29-45,共17页植物通讯（英文）

基　　金：funded by the National Natural Science Foundation of China(32271905 and 32270382);the Zhejiang Provincial Natural Science Foundation of China under grants LY23C160001,LY18C050005,LY19C150005,and LY19C160001;the Opening Project of Zhejiang Provincial Key Laboratory of Forest Aromatic Plant-Based Healthcare Functions(2022E10008);the Open Foundation of State Key Laboratory of Subtropical Silviculture,Zhejiang A&F University(KF201708);the Major Increase or Decrease Program in The Central Finance Level(grant 2060302);Zhejiang Provincial Key Research&Development Project grants(2017C02011,2018C02030).

摘　　要：Taxus leaves provide the raw industrial materials for taxol,a natural antineoplastic drug widely used in the treatment of various cancers.However,the precise distribution,biosynthesis,and transcriptional regulation of taxoids and other active components in Taxus leaves remain unknown.Matrix-assisted laser desorption/ionization–mass spectrometry imaging analysis was used to visualize various secondary metabolites in leaf sections of Taxus mairei,confirming the tissue-specific accumulation of different active metabolites.Single-cell sequencing was used to produce expression profiles of 8846 cells,with a median of 2352 genes per cell.Based on a series of cluster-specific markers,cells were grouped into 15 clusters,suggesting a high degree of cell heterogeneity in T.mairei leaves.Our data were used to create the first Taxus leaf metabolic single-cell atlas and to reveal spatial and temporal expression patterns of several secondary metabolic pathways.According to the cell-type annotation,most taxol biosynthesis genes are expressed mainly in leaf mesophyll cells;phenolic acid and flavonoid biosynthesis genes are highly expressed in leaf epidermal cells(including the stomatal complex and guard cells);and terpenoid and steroid biosynthesis genes are expressed specifically in leaf mesophyll cells.A number of novel and cell-specific transcription factors involved in secondary metabolite biosynthesis were identified,including MYB17,WRKY12,WRKY31,ERF13,GT_2,and bHLH46.Our research establishes the transcriptional landscape of major cell types in T.mairei leaves at a single-cell resolution and provides valuable resources for studying the basic principles of cell-type-specific regulation of secondary metabolism.

关 键 词：scRNA-seq taxol biosynthesis Taxus mairei secondary metabolism transcription regulation 

分 类 号：Q94[生物学—植物学]