Combining single-cell RNA sequencing with spatial transcriptome analysis reveals dynamic molecular maps of cambium differentiation in the primary and secondary growth of trees  

作　　者：Renhui Li Zhifeng Wang Jia-Wei Wang Laigeng Li 

机构地区：[1]National Key Laboratory of Plant Molecular Genetics and CAS Center for Excellence in Molecular Plant Sciences,Institute of Plant Physiology and Ecology,Chinese Academy of Sciences,Shanghai 200032,China [2]University of the Chinese Academy of Sciences,Beijing 100049,China [3]School of Life Science and Technology,ShanghaiTech University,Shanghai 201210,China

出　　处：《Plant Communications》2023年第5期46-62,共17页植物通讯（英文）

基　　金：supported by the National Natural Science Foundation of China(32130072);the Chinese Academy of Sciences’Strategic Priority Research Program(XDB27020104);the National Key Research and Development Program(2021YFD2200204).

摘　　要：Primary and secondary growth of the tree stem are responsible for corresponding increases in trunk height and diameter.However,our molecular understanding of the biological processes that underlie these two types of growth is incomplete.In this study,we used single-cell RNA sequencing and spatial transcriptome sequencing to reveal the transcriptional landscapes of primary and secondary growth tissues in the Populus stem.Comparison between the cell atlas and differentiation trajectory of primary and secondary growth revealed different regulatory networks involved in cell differentiation from cambium to xylem precursors and phloem precursors.These regulatory networks may be controlled by auxin accumulation and distribution.Analysis of cell differentiation trajectories suggested that vessel and fiber development followed a sequential pattern of progressive transcriptional regulation.This research provides new insights into the processes of cell identity and differentiation that occur throughout primary and secondary growth of tree stems,increasing our understanding of the cellular differentiation dynamics that occur during stemgrowth in trees.

关 键 词：scRNA-seq spatial transcriptome primary growth secondary growth secondary cell wall SCW POPULUS 

分 类 号：Q94[生物学—植物学]