Genome-wide scan for oil quality reveals a coregulation mechanism of tocopherols and fatty acids in soybean seeds  

作　　者：Danni Chu Zhifang Zhang Yang Hu Chao Fang Xindan Xu Jia Yuan Jinsong Zhang Zhixi Tian Guodong Wang 

机构地区：[1]State Key Laboratory of Plant Genomics,Institute of Genetics and Developmental Biology,The Innovative Academy of Seed Design,Chinese Academy of Sciences,Beijing 100101,China [2]State Key Laboratory of Plant Cell and Chromosome Engineering,Institute of Genetics and Developmental Biology,The Innovative Academy of Seed Design,Chinese Academy of Sciences,Beijing 100101,China [3]College of Advanced Agricultural Sciences,University of Chinese Academy of Sciences,Beijing 100039,China [4]Present address:Department of Plant Biology,Michigan State University,East Lansing,MI 48824,USA

出　　处：《Plant Communications》2023年第5期234-245,共12页植物通讯（英文）

基　　金：financially supported by the National Key Research and Development Program of China(2021YFF1001200 and 2018YFA0900600);the Chinese Academy of Science(XDA24040202);the State Key Laboratory of Plant Genomics of China(grant no.SKLPG2016A-13).

摘　　要：Tocopherols(vitamin E)play essential roles in human health because of their antioxidant activity,and plantderived oils are the richest sources of tocopherols in the human diet.Although soybean(Glycinemax)is one of themain sources of plant-derived oil and tocopherol in the world,the relationship between tocopherol and oil in soybean seeds remains unclear.Here,we focus on dissecting tocopherol metabolism with the longterm goal of increasing a-tocopherol content and soybean oil quality.We first collected tocopherol and fatty acid profiles in a soybean population(>800 soybean accessions)and found that tocopherol content increased during soybean domestication.A strong positive correlation between tocopherol and oil content was also detected.Five tocopherol pathway–related lociwere identified using a metabolite genome-wide association study strategy.Genetic variations in three tocopherol pathway genes were responsible for total tocopherol content and composition in the soybean population through effects on enzyme activity,mainly caused by non-conserved amino acid substitution or changes in gene transcription level.Moreover,the fatty acid regulatory transcription factor GmZF351 directly activated tocopherol pathway gene expression,increasing both fatty acid and tocopherol contents in soybean seeds.Our study reveals the functional differentiation of tocopherol pathway genes in soybean populations and provides a framework for development of new soybean varieties with high a-tocopherol content and oil quality in seeds.

关 键 词：SOYBEAN fatty acid TOCOPHEROL vitamin E mGWAS oil quality 

分 类 号：Q94[生物学—植物学]