Suppression of ZEAXANTHIN EPOXIDASE 1 restricts stripe rust growth in wheat  

作　　者：Chao-Yan Chang Shu-Xian Yang Mei-Qi Zhang Yue-Ting Guo Xiao-Ming Li Yan Yan Ci-Hang Ding Ke-Xin Niu Meng-Lu Wang Qin-Quan Li Junli Zhang Xuebin Zhang Shisheng Chen Chaojie Xie Zhongfu Ni Qixin Sun Jin-Ying Gou 

机构地区：[1]Key Laboratory of Crop Heterosis and Utilization(MOE)and Beijing Key Laboratory of Crop Genetic Improvement,China Agricultural University,Beijing 100193,China [2]School of Life Sciences,Fudan University,Shanghai 200438,China [3]State Key Laboratory of Crop Stress Adaptation and Improvement,Henan Joint International Laboratory for Crop Multi-Omics Research,School of Life Sciences,Henan University,Jinming Road,Kaifeng 475004,China [4]Peking University Institute of Advanced Agricultural Sciences,Shandong Laboratory of Advanced Agricultural Sciences at Weifang,Weifang,Shandong 261000,China

出　　处：《Plant Communications》2023年第5期295-308,共14页植物通讯（英文）

基　　金：supported by the National Key Research and Development Program(2022YFF1001501);the National Natural Science Foundation of China(31972350);the Chinese Universities Scientific Fund(2022TC174);the financial support from an open project of the State Key Laboratory of Crop Stress Adaptation and Improvement in Henan University.

摘　　要：Reducing losses caused by pathogens is an effective strategy for stabilizing crop yields.Daunting challenges remain in cloning and characterizing genes that inhibit stripe rust,a devastating disease of wheat(Triticum aestivum)caused by Puccinia striiformis f.sp.tritici(Pst).We found that suppression of wheat zeaxanthin epoxidase 1(ZEP1)increased wheat defense against Pst.We isolated the yellow rust slower 1(yrs1)mutant of tetraploid wheat in which a premature stop mutation in ZEP1-B underpins the phenotype.Genetic analyses revealed increased H_(2)O_(2) accumulation in zep1 mutants and demonstrated a correlation between ZEP1 dysfunction and slower Pst growth in wheat.Moreover,wheat kinase START 1.1(WKS1.1,Yr36)bound,phosphorylated,and suppressed the biochemical activity of ZEP1.A rare natural allele in the hexaploid wheat ZEP1-B promoter reduced its transcription and Pst growth.Our study thus identified a novel suppressor of Pst,characterized its mechanism of action,and revealed beneficial variants for wheat disease control.This work opens the door to stacking wheat ZEP1 variants with other known Pst resistance genes in future breeding programs to enhance wheat tolerance to pathogens.

关 键 词：WHEAT stripe rust reactive oxygen species zeaxanthin epoxidase 

分 类 号：S512.1[农业科学—作物学]