贵州六盘水地区大叶黄杨叶枯病病原菌鉴定  

Pathogenic Identification of Leaf Blight on Euonymus japonicus in Guizhou Province

作　　者：王瑞仙 田霜 鲁武锋 方英艺 张孝敬 安传相 谭莉 杨友联 Wang Rui-xian(Fenggang Agriculture and Rural Bureau,Fenggang,Guizhou 564200)

机构地区：[1]凤冈县农业农村局,贵州凤冈564200 [2]六盘水师范学院生物科学与技术学院,贵州六盘水553004

出　　处：《农业灾害研究》2023年第8期37-40,64,共5页Journal of Agricultural Catastrophology

基　　金：贵州省教育厅基金项目(黔教合KY字[2015]503号)。

摘　　要：对贵州省六盘水市采集到疑似叶枯病的大叶黄杨病叶进行病原菌鉴定,采用孢子稀释法共分离到2类纯化菌株。对2类纯化菌株进行科赫氏验证,确定其为大叶黄杨叶枯病致病菌。通过形态学结合多基因(ITS、β-tubulin、tef-1)分子系统学鉴定,表明2类纯化菌株均为拟盘多毛孢属(Pestalotiopsis),其中菌株LPSU2015001鉴定结果为Pestalotiopsis intermedia,菌株LPSU2015002暂定为Pestalotiopsis sp.,这在大叶黄杨叶枯病防治及植物育种方面具有重要的实践意义,且丰富了真菌资源。The diseased leaves of Euonymus japonicus collected from Liupanshui District,Guizhou Province were collected,and in order to identify the species,2 purified class were obtained by spore dilution method.2 representative strains were selected for pathogenicity test and identified as pathogenic fungi of leaf blight on Euonymus japonicus by Koch's law.Based on the morphological characters and polygene molecular systematics(ITS、β-tubulin、tef-1),three strains belong to Pestalotiopsis.Strain LPSU2015001represent Pestalotiopsis intermedia,Strain LPSU2015002 was identified as Pestalotiopsis sp..The outcome will have important practical implications for prevent the pathogenic fungi of leaf blight and enrich the fungal resource.

关 键 词：大叶黄杨 叶枯病 拟盘多毛孢属 多基因分子系统学 

分 类 号：S763.7[农业科学—森林保护学]