海南省136株耐药结核分枝杆菌寡核苷酸基因分型和耐药性分析  

Spoligotyping and drug resistance analysis of 136 drug-resistant Mycobacterium tuberculosis strains in Hainan Province

作　　者：黄静静[1] 侯萍 曾祥洁[1] 柯芊羽 罗兴雄[1] HUANG Jingjing;HOU Ping;ZENG Xiangjie;KE Qianyu;LUO Xingxiong(Hainan Provincial Center for Disease Control and Prevention of Infectious Disease Prevention and Control of Tuberculosis Prevention and Control,Haikou,Hainan 570203,China)

机构地区：[1]海南省疾病预防控制中心传染病预防控制所结核病预防控制科,海南海口570203

出　　处：《中国热带医学》2023年第9期977-982,共6页China Tropical Medicine

摘　　要：目的 通过对2022年收集的海南省136株耐药结核分枝杆菌进行基因分型研究,了解和掌握海南省不同耐药结核分枝杆菌基因型的流行情况及耐药率,为海南省结核病防治策略提供科学依据。方法 收集海南省结核分枝杆菌耐药菌株136株,采用间隔区寡核苷酸分型技术(Spoligotyping)对结核分枝杆菌临床分离株进行基因分型,并对不同基因型结核分枝杆菌的耐药率进行统计学分析。结果 136株耐药结核分枝杆菌中北京型占54.41%(74/136),非北京型占27.94%(38/136),新发现基因型占17.65%(24/136)。北京型中包含SIT1和SIT269两种基因型,占比分别为52.94%(72/136)、1.47%(2/136)。在非北京型中,T型(T1、T2、T3)占21.32%(29/136),U型占6.62%(9/136)。基因分型聚类分析结果:分为北京型和非北京型两个大类以及一些散在的新型基因型。Spoligotyping分型成簇分析结果:136株耐药菌株分为3簇,成簇率为75.74%(103/136)。北京型和非北京型的单耐药、多耐药、耐多药、广泛耐药比率分别为41.89%(31/74)、13.51%(10/74)、24.33%(18/74)、20.27%(15/74)和36.84%(14/38)、15.79%(6/38)、26.32%(10/38)、21.05%(8/38),经过卡方检验,北京型和非北京型两者之间以上各耐药率差异均无统计学意义(P>0.05)。结论 海南省结核分枝杆菌基因型呈基因多态性,其主要流行菌株基因型为北京型中的SIT1型,应加强该基因型结核分枝杆菌监测工作。Objective In order to understand and master the prevalence of different genotypes and the rate of different drug-resistant Mycobacterium tuberculosis genotypes in Hainan Province,136 drug-resistant Mycobacterium tuberculosis strains collected in Hainan province in 2022 were genotyped,and to provide scientific basis for tuberculosis prevention and control strategy in Hainan Province.Methods A total of 136 drug-resistant Mycobacterium tuberculosis strains were collected in Hainan Province.The clinical isolates were genotyped using the Spoligotyping technique,and the drug resistance rates of different genotypes of Mycobacterium tuberculosis were statistically analyzed.Results Among the 136 strains of drugresistant Mycobacterium tuberculosis,54.41%(74/136)belonged to the Beijing types,27.94%(38/136)to non-Beijing types and newly identified genotypes accounted for 17.65%(24/136).The Beijing type included two genotypes,SIT1 and SIT269 genotypes,accounting for 52.94%(72/136)and 1.47%(2/136)respectively.Among the non-Beijing genotypes,the T type(T1,T2,T3)accounted for 21.32%(29/136),the U type accounted for 6.62%(9/136).Clustering analysis of genotyping results revealed two major clusters,Beijing type and non-Beijing type,as well as several scattered novel genotypes.Clustering analysis of Spoligotyping results classified the 136 drug-resistant strains into 3 clusters,with a clustering rate of 75.74%(103/136).The rates of mono-resistance(MR),poly-resistance(PR),multi-drug resistance(MDR),and other types of drug resistance in Beijing type and non-Beijing type were 41.89%(31/74),13.51%(10/74),24.33%(18/74),20.27%(15/74)and 36.84%(14/38),15.79%(6/38),26.32%(10/38),21.05%(8/38)respectively.Chi-square test results showed no statistically significant differences in drug resistance rates between the Beijing and non-Beijing types(P>0.05).Conclusion The genotype of Mycobacterium tuberculosis in Hainan Province showed genetic polymorphism,with the main epidemic genotype being SIT1 in the Beijing type.Monitoring of Mycobacterium tub

关 键 词：结核分枝杆菌 间隔区寡核苷酸分型技术 基因分型 耐药性 海南省 

分 类 号：R378.911[医药卫生—病原生物学]