黄蜀葵花药用部位与非药用部位化学成分的比较分析  

Comparative Study on Chemical Constituents of Medicinal and Non-medicinal Parts of Flos Abelmoschus manihot

作　　者：殷圣鑫 蔡芷辰[1] 陈翠花 魏丽芳 梅余琪 刘训红[1,3] 邹立思 袁嘉欢[1] 吴楠 葛海涛 王殿广[4] 王丹丹 YIN Sheng-xin;CAI Zhi-chen;CHEN Cui-hua;WEI Li-fang;MEI Yu-qi;LIU Xun-hong;ZOU Li-si;YUAN Jia-huan;WU Nan;GE Hai-tao;WANG Dian-guang;WANG Dan-dan(School of Pharmacy,Nanjing University of Chinese Medicine,Nanjing 210023,China;Lianyungang Food and Drug Inspection Center,Lianyungang 222069,China;Jiangsu Province Engineering Research Center of Classical Prescription,Nanjing 210023,China;Suzhong Pharmaceutical Group Co.,Ltd.,Taizhou 225500,China)

机构地区：[1]南京中医药大学药学院,南京210023 [2]连云港市食品药品检验检测中心,江苏连云港222069 [3]江苏省经典名方工程研究中心,南京210023 [4]江苏苏中药业集团股份有限公司,江苏泰州225500

出　　处：《中国药学杂志》2023年第14期1276-1286,共11页Chinese Pharmaceutical Journal

基　　金：江苏高校优势学科建设工程项目资助(YSXK-2014);江苏高校品牌专业建设工程项目资助(PPZY2015A070)。

摘　　要：目的建立基于超快速液相色谱-三重四极杆/线性离子阱质谱(UFLC-QTRAP-MS/MS)同时测定黄蜀葵花药用部位与非药用部位中黄酮、有机酸、核苷和氨基酸类38种化学成分含量的方法,对黄蜀葵花药用部位(花冠)与非药用部位(花萼、雄蕊、雌蕊)中化学成分的含量进行比较,并结合多元统计分析(PCA,OPLS-DA)探究二者化学成分的差异。方法采用XBridge■C18(4.6 mm×100 mm,3.5μm)色谱柱分离,以0.1%甲酸水-甲醇:乙腈(1∶1)为流动相,梯度洗脱,流速0.5 mL·min^(-1),柱温30℃,选择多反应离子监测(MRM)模式测定(黄酮和有机酸类成分采用负离子模式,核苷和氨基酸类成分采用正离子模式进行检测)。结果含量测定结果显示,黄酮类成分集中在药用部位花冠,含量为60905.55~69851.44μg·g^(-1),非药用部位黄酮类成分含量为2988.96~4813.60μg·g^(-1);有机酸、核苷和氨基酸类成分在非药用部位中含量较高。多元统计分析结果表明,黄蜀葵花药用部位与非药用部位区别明显,筛选出区分二者的5种差异化合物(槲皮素-3-O-刺槐糖苷、金丝桃苷、异槲皮苷、棉皮素-8-葡萄糖醛酸苷、槲皮素-3′-O-葡萄糖苷),且药用部位花冠中5种差异化合物的含量均显著高于非药用部位。结论实验验证了黄蜀葵花药材以花冠入药的科学性和合理性,非药用部位的混入会影响其药材质量,因此在药材生产、使用过程中,必须严格控制非药用部位混入,以确保黄蜀葵花药材的优质和质量均一。该研究可为黄蜀葵花药材的合理采收和市场标准的严格规范提供科学依据,亦为黄蜀葵花药材内在质量的综合评价和全面控制提供方法参考。OBJECTIVE To establish a comprehensive analytical method based on ultra-fast liquid chromatography coupled with triple quadrupole/linear ion trap tandem mass spectrometry(UFLC-QTRAP-MS/MS)for simultaneous determination of content of 38 chemical constituents in medicinal and non-medicinal parts of Flos Abelmoschus manihot,including flavonoids,organic acids,nucleosides and amino acids.METHODS The chromatographic separation was performed on a XBridge■C18 column(4.6 mm×100 mm,3.5μm)at 30℃with a gradient elution of 0.1%formic acid water-methanol∶acetonitrile(1∶1)at a flow rate of 0.5 mL·min^(-1).The constituents were detected under multiple-reaction monitoring(MRM)mode(flavonoids and organic acids were detected by negative ion mode,nucleosides and amino acids were detected by positive ion mode).RESULTS The result of content determination showed that flavonoids were concentrated in the corolla of medicinal part.The content of flavonoids in corolla and non-medicinal parts of Flos Abelmoschus manihot were 60905.55-69851.44μg·g^(-1)and 2988.96-4813.60μg·g^(-1),respectively.Organic acids,nucleosides,and amino acids were more abundant in non-medicinal parts.Multivariate statistical analysis showed that there were significant differences between medicinal part and non-medicinal parts of Flos Abelmoschus manihot.Five differential constituents(quercetin 3-O-robinobioside,hyperin,isoquercetin,hibifolin,quercetin 3′-O-glucoside)were screened out to distinguish them,and the contents of five differential constituents in the corolla of medicinal part were significantly higher than those of non-medicinal parts.CONCLUSION The experiment verified the scientificity and rationality of using the corolla as Abelmoschi Corolla.The mixing of non-medicinal parts will affect the quality of medicinal materials.Therefore,the mixing of non-medicinal parts must be strictly controlled during the process of production and use of medicinal materials to ensure the high quality and uniform quality of Abelmoschi Corolla.This study can

关 键 词：黄蜀葵花 药用部位 非药用部位 超快速液相色谱-三重四极杆/线性离子阱质谱 多元统计分析 

分 类 号：R282[医药卫生—中药学]