温和灸对原发性痛经模型大鼠子宫微循环及血液流变学的影响  

Effects of Mild Moxibustion on Uterine Microcirculation and Hemorheology in Rat Model of Primary Dysmenorrhea

作　　者：郭莎 李雪梅 任奎羽 陈兆恒 杨莎[1] GUO Sha;LI Xuemei;REN Kuiyu;CHEN Zhaoheng;YANG Sha(College of Acupuncture,Moxibustion and Tuina,Chengdu University of Traditional Chinese Medicine,Chengdu 610075,China;Nanchong Mental Health Center,Nanchong 637000,China)

机构地区：[1]成都中医药大学针灸推拿学院,成都610075 [2]南充精神卫生中心,南充637000

出　　处：《世界中医药》2023年第17期2435-2440,共6页World Chinese Medicine

基　　金：国家自然基金青年科学基金项目(82004487);四川省教育厅科研一般项目(18ZB0201)。

摘　　要：目的:探讨温和灸对原发性痛经大鼠子宫微循环的影响。方法:将40只雌性SD大鼠随机分为空白组、空白加艾灸组、模型组、艾灸组,采用苯甲酸雌二醇联合缩宫素造模法制备大鼠子宫痛经模型,选择关元和三阴交对痛经大鼠进行温和灸治疗,分别观察测定大鼠扭体反应、子宫组织形态学、子宫微血管管径和血液流变学的变化情况。结果:除空白组和空白加艾灸组外,模型组与艾灸组均出现扭体反应,提示痛经大鼠模型制造成功;与空白组及空白加艾灸组比较,模型组子宫横径明显增加(P<0.05),微血管管径明显收缩(P<0.05)。与模型组比较,艾灸组子宫横径减少明显(P<0.05),微血管管径显著扩张(P<0.05);与空白组比较,模型组与艾灸组全血黏度各切变度、红细胞刚性指数、红细胞变形指数、红细胞沉降率方程K值及红细胞压积降低(P<0.05)。与模型组比较,艾灸组全血黏度各切变度、红细胞刚性指数、红细胞变形指数、红细胞沉降率方程K值及红细胞压积有升高的趋势(P>0.05);空白组与空白加艾灸组各项指标差异均无统计学意义(均P>0.05)。结论:温和灸对缩宫素所致大鼠实验性痛经有一定抑制作用,其抗痛经作用可能与改善子宫水肿及血液流变学,缓解子宫微循环障碍有关。Objective:To investigate the effect of mild moxibustion on uterine microcirculation in the rat model of primary dysmenorrhea(PD).Methods:Forty female SD rats were randomized into blank,blank+moxibustion,model,and model+moxibustion groups.The rat model of PD was prepared by estradiol benzoate combined with oxytocin.The mild moxibustion was performed at Guanyuan(CV4)and Sanyinjiao(SP6).The writhing response,uterine histomorphology,uterine microvascular diameter,and hemorheology were observed and measured respectively.Results:Except the blank group and blank+moxibustion group,the model group and model+moxibustion group showed writhing response,indicating that the PD model was successfully established.Compared with the blank group and blank+moxibustion group,the model group showed increased transverse diameter of uterus(P<0.05)and decreased microvascular diameter(P<0.05).Compared with the model group,the model+moxibustion group showed decreased transverse diameter of uterus(P<0.05)and increased microvascular diameter(P<0.05).Compared with the blank group,the model group and model+moxibustion group showed decreased whole blood viscosity at different shear rates,erythrocyte rigidity index,erythrocyte deformability index,K value of erythrocyte sedimentation equation,and hematocrit(P<0.05).Compared with the model group,the model+moxibustion group showed increased whole blood viscosity at different shear rates,erythrocyte rigidity index,erythrocyte deformability index,K value of erythrocyte sedimentation equation,and hematocrit(P>0.05).There were no significant differences in the indexes between the blank group and blank+moxibustion group(P>0.05).Conclusion:Mild moxibustion can inhibit dysmenorrhea induced by oxytocin in rats by alleviating uterine edema,improving uterine hemorheology,and alleviating uterine microcirculation disorder.

关 键 词：原发性痛经 温和灸 子宫微循环 血液流变学 大鼠 痛经模型 关元 三阴交 

分 类 号：R245[医药卫生—针灸推拿学]