丙泊酚诱导新生大鼠少突胶质细胞的凋亡:基于激活caspase家族蛋白和抑制抗凋亡程序  

Mechanism of propofol-induced apoptosis in neonatal rat oligodendrocytes

作　　者：纪雪霞 朱毅[1] 张登文 蔡宇晶 周国斌 JI Xuexia;ZHU Yi;ZHANG Dengwen;CAI Yujing;ZHOU Guobin(Department of Anesthesiology,Guangdong Provincial People's Hospital(Guangdong Academy of Medical Sciences),Southern Medical University,Guangzhou 510080,China)

机构地区：[1]南方医科大学附属广东省人民医院(广东省医学科学院)麻醉科,广东广州510080

出　　处：《南方医科大学学报》2023年第10期1771-1775,共5页Journal of Southern Medical University

基　　金：广州市科技计划项目(202201010853,202201010852)。

摘　　要：目的探讨丙泊酚诱导少突胶质细胞凋亡的分子机制。方法采用随机数字表法将45只7 d SD乳鼠分为对照组(n=15)、脂肪乳剂组(n=15)和50 mg/kg丙泊酚组(n=15)。单次腹腔注射丙泊酚,0.4 mL/只(体积不足者用生理盐水补齐)。对照组和脂肪乳剂组腹腔注射等体积的生理盐水和中长链脂肪乳。丙泊酚腹腔注射麻醉8 h后,采用qPCR和Westernblot检测不同组别caspase-3、caspase-8、caspase-9 mRNA和蛋白表达水平;检测NGF mRNA和P-PI3K/P-PAkt表达水平。结果与对照组相比,丙泊酚组caspase-3、caspase-8、caspase-9 mRNA和蛋白表达水平升高(P<0.05);NGF mRNA和P-PI3K/P-PAkt表达水平降低(P<0.05)。结论丙泊酚通过激活凋亡程序中caspase家族蛋白成员和抑制抗凋亡程序,诱导少突胶质细胞的凋亡。Objective To explore the molecular mechanism of propofol-induced apoptosis in oligodendrocytes.Methods Forty-five neonatal(7 days old)SD rats were randomized into 3 groups(n=15)for a single intraperitoneal injection of saline(control),long chain fat emulsion,or propofol(50 mg/kg).Eight hours after the injection,the rats were examined for mRNA and protein expressions of caspase-3,caspase-8,and caspase-9 in the brain tissues using qPCR and Western blotting,and the expression levels of nerve growth factor(NGF)mRNA and P-PI3K/P-PAkt were also detected.Results Compared with those in the control group,the neonatal rats with propofol injection showed significantly up-regulated mRNAand protein expressions of caspase-3,caspase-8,and caspase-9 in the brain tissue(P<0.05)with significantly down-regulated expressions of NGF mRNA and P-PI3K/P-PAkt(P<0.05).Conclusion Propofol induces apoptosis in oligodendrocytes by activating the caspase family protein members involved in triggering cell apoptosis and inhibiting the anti-apoptosis mechanism.

关 键 词：丙泊酚 少突胶质细胞 CASPASE家族 抗凋亡程序 

分 类 号：R614[医药卫生—麻醉学]