Dissecting peri-implantation development using cultured human embryos and embryo-like assembloids  

作　　者：Zongyong Ai Baohua Niu Yu Yin Lifeng Xiang Gaohui Shi Kui Duan Sile Wang Yingjie Hu Chi Zhang Chengting Zhang Lujuan Rong Ruize Kong Tingwei Chen Yixin Guo Wanlu Liu Nan Li Shumei Zhao Xiaoqing Zhu Xuancheng Mai Yonggang Li Ze Wu Yi Zheng Jianping Fu Weizhi Ji Tianqing Li 

机构地区：[1]State Key Laboratory of Primate Biomedical Research,Institute of Primate Translational Medicine,Kunming University of Science and Technology,Kunming,Yunnan,China [2]Yunnan Key Laboratory of Primate Biomedical Research,Kunming,Yunnan,China [3]Yunnan Provincial Academy of Science and Technology,Kunming,Yunnan,China [4]Department of Reproductive Medicine,The First People’s Hospital of Yunnan Province,Kunming,Yunnan,China [5]Zhejiang University-University of Edinburgh Institute(ZJU-UoE Institute),Zhejiang University School of Medicine,International Campus,Zhejiang University,Haining,Zhejiang,China [6]Department of Mechanical Engineering,University of Michigan,Ann Arbor,MI,USA [7]Department of Biomedical Engineering,University of Michigan,Ann Arbor,MI,USA [8]Department of Cell&Developmental Biology,University of Michigan Medical School,Ann Arbor,MI,USA

出　　处：《Cell Research》2023年第9期661-678,共18页细胞研究（英文版）

基　　金：This work was supported by the National Key R&D Program of China(2022YFA1103100 and 2021YFA0805700);the National Natural Science Foundation of China(32130034,82192874,32100655 and 82160294);the National Key R&D Program of China(2020YFA0112700);the Major Basic Research Project of Science and Technology of Yunnan(202102AA100007,202001BC070001 and 2019FY002);the Joint Special Funds for the Department of Science and Technology of Yunnan Province‑Kunming Medical University(202101AY070001-260 and 202301AY070001-298).

摘　　要：Studies of cultured embryos have provided insights into human peri-implantation development.However,detailed knowledge of peri-implantation lineage development as well as underlying mechanisms remains obscure.Using 3D-cultured human embryos,herein we report a complete cell atlas of the early post-implantation lineages and decipher cellular composition and gene signatures of the epiblast and hypoblast derivatives.In addition,we develop an embryo-like assembloid(E-assembloid)by assembling naive hESCs and extraembryonic cells.Using human embryos and E-assembloids,we reveal that WNT,BMP and Nodal signaling pathways synergistically,but functionally differently,orchestrate human peri-implantation lineage development.Specially,we dissect mechanisms underlying extraembryonic mesoderm and extraembryonic endoderm specifications.Finally,an improved E-assembloid is developed to recapitulate the epiblast and hypoblast development and tissue architectures in the pregastrulation human embryo.Our findings provide insights into human peri-implantation development,and the E-assembloid offers a useful model to disentangle cellular behaviors and signaling interactions that drive human embryogenesis.

关 键 词：IMPLANTATION architectures INSIGHT 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]