Nm-Mut-seq:a base-resolution quantitative method for mapping transcriptome-wide 2′-O-methylation  

作　　者：Li Chen Li-Sheng Zhang Chang Ye Huiqing Zhou Bei Liu Boyang Gao Zixin Deng Changming Zhao Chuan He Bryan C.Dickinson 

机构地区：[1]Department of Chemistry,The University of Chicago,Chicago,IL,USA [2]Department of Geriatrics,Zhongnan Hospital of Wuhan University,School of Pharmaceutical Sciences,Wuhan University,Wuhan,Hubei,China [3]Howard Hughes Medical Institute,The University of Chicago,Chicago,IL,USA [4]Department of Biochemistry and Molecular Biology,The University of Chicago,Chicago,IL,USA

出　　处：《Cell Research》2023年第9期727-730,共4页细胞研究（英文版）

基　　金：This work was supported by the National Human Genome Research Institute(RM1 HG008935 to C.H.and B.C.D.);the National Institute of Mental Health(R01 MH122142 to B.C.D.)of the National Institutes of Health(NIH);the University of Chicago Medicine Comprehensive Cancer Center(P30 CA14599)and the international postdoctoral exchange fellowship program(20190048)(L.C.);The evolved RT biochemistry work was partially supported by the National Key R&D Program of China(2018YFA0903200 to C.Z.).C.H.is an investigator at Howard Hughes Medical Institute.We thank Dr.Qing Dai for his help and suggestions on library construction.

摘　　要：Dear Editor,2'-O-methylation(Nm)(Fig.1a)is a prevalent post-transcriptional RNA modification present in many cellular RNAs and plays critical roles in modulating both physical properties and functions of eukaryotic RNA.Studies of Nm modifications in RNA havelong been hampered by a lack of effective mapping methods.

关 键 词：mapping methods BASE 

分 类 号：Q2[生物学—细胞生物学]