外源6-BA和KH_(2)PO_(4)对花后受渍小麦根系抗氧化酶和无氧呼吸酶活性的影响  

Effects of exogenous 6-BA and KH_(2)PO_(4)on antioxidant enzymes and anaerobic respiratory enzymes activities in wheat roots under waterlogging at post-flowering stage

作　　者：耿兵婕 叶苗苗 陈研 王孟昌 马尚宇[1,2] 黄正来 张文静[1] 樊永惠 GENG Bingjie;YE Miaomiao;CHEN Yan;WANG Mengchang;MA Shangyu;HUANG Zhenglai;ZHANG Wenjing;FAN Yonghui(Key Laboratory of Wheat Biology and Genetic Breeding in Southern Huanghuai,Ministry of Agriculture and Rural Affairs,College of Agronomy,Anhui Agricultural University,Hefei 230036,China;Collaborative Innovation Center of Modern Crop Production,Jiangsu Province,Nanjing 210095,China)

机构地区：[1]安徽农业大学农学院,农业农村部黄淮南部小麦生物学与遗传育种重点实验室,安徽合肥230036 [2]江苏省现代作物生产协同创新中心,江苏南京210095

出　　处：《浙江农业学报》2023年第10期2275-2285,共11页Acta Agriculturae Zhejiangensis

基　　金：安徽省高等学校科学研究项目(2022AH050906);国家自然科学基金(31801287)。

摘　　要：为研究不同外源修复物质对受渍小麦根系生长发育的影响,以扬麦18为材料,在开花期设置渍水9 d(W9)和正常灌溉(W0)处理,两处理分别叶面喷施蒸馏水(T0)、0.01 mmol·L^(-1)6-BA(T1)、0.3%KH_(2)PO_(4)(T2)、0.01 mmol·L^(-1)6-BA和0.3%KH_(2)PO_(4)复配溶液(T3),研究不同外源物质对小麦根系抗氧化酶和无氧呼吸酶活性的影响。结果表明,W0条件下,喷施0.3%KH_(2)PO_(4)和0.01 mmol·L 6-BA可以改善根系中超氧化物歧化酶(SOD)、过氧化物酶(POD)、乙醇脱氢酶(ADH)活性,提高小麦千粒重,进而提高产量;W9处理中,小麦0~60 cm土层根系中SOD、ADH、乳酸脱氢酶(LDH)活性均有不同程度降低,而POD活性、丙二醛(MDA)含量显著提高,而喷施0.3%KH_(2)PO_(4)和0.01 mmol·L 6-BA显著提高了各土层根系SOD、ADH、LDH活性,降低POD活性和MDA含量,其中,喷施0.01 mmol·L^(-1)6-BA和0.3%KH_(2)PO_(4)复配剂显著提高了小麦根系抗氧化能力和无氧呼吸能力,其成熟期千粒重和产量分别比渍水处理提高23.34%、37.43%。To investigate the effects of different exogenous substances on the growth and development of root system in waterlogged wheat plants,the cultivar Yangmai 18 was used as the experimental material.The plants were subjected to either waterlogging treatment for 9 days(W9)or normal irrigation(W0)during the flowering stage.Different exogenous substances were sprayed on the foliage,including distilled water(T0),0.01 mmol·L^(-1)6-BA(T1),0.3%KH_(2)PO_(4)(T2),and a mixture of 0.01 mmol·L^(-1)6-BA and 0.3%KH_(2)PO_(4)(T3).The effects of these exogenous substances on the activities of antioxidant enzymes and anaerobic respiratory enzymes in wheat root system were studied.The results showed that under W0 conditions,foliar application of 0.3%KH_(2)PO_(4)and 0.01 mmol·L^(-1)6-BA could improve the activities of superoxide dismutase(SOD),peroxidase(POD),and alcohol dehydrogenase(ADH)in the root system,increase the 1000-grain weight of wheat,and ultimately enhance the yield.Under W9 treatment,the activities of SOD,ADH,and lactate dehydrogenase(LDH)in the wheat root system in the 0-60 cm soil layer were significantly reduced to varying degrees,while the activity of POD and the content of malondialdehyde(MDA)were significantly increased.However,the application of 0.3%KH_(2)PO_(4)and 0.01 mmol·L^(-1)6-BA significantly increased the activities of SOD,ADH,and LDH in the root system in each soil layer,reduced the activity of POD and the content of MDA.In particular,the combination of 0.01 mmol·L^(-1)6-BA and 0.3%KH_(2)PO_(4)significantly improved the antioxidant capacity and anaerobic respiration capacity of the wheat root system,resulting in 23.34%and 37.43%increase in 1000-grain weight and yield compared to the waterlogged treatment.

关 键 词：小麦 根系 渍水 抗氧化酶 无氧呼吸酶 

分 类 号：S512.1[农业科学—作物学]