基质辅助激光解吸电离飞行时间质谱在微生物鉴定中的研究进展  

Research progress of matrix-assisted laser desorptionionization time-of-flight mass spectrometry in microbialidentification

作　　者：尹盼盼 白雯静 李海燕 彭腾腾 石晓峰 YIN Panpan;BAI Wenjing;LI Haiyan;PENG Tengteng;SHI Xiaofeng(College of Pharmacy,Gansu University of Chinese Medicine,Lanzhou 730000,Gansu,China;Gansu Provincial Academic Institute for Medical Research,Lanzhou 730000,Gansu,China;Lanzhou Institute of Food and Drug Inspection and Testing,Lanzhou 730000,Gansu,China)

机构地区：[1]甘肃中医药大学药学院,甘肃兰州730000 [2]甘肃省医学科学研究院,甘肃兰州730000 [3]兰州市食品药品检验检测研究院,甘肃兰州730000

出　　处：《微生物学通报》2023年第10期4655-4666,共12页Microbiology China

基　　金：2022年度甘肃省陇原青年创新创业人才项目;2021年甘肃省科技重大项目(21ZD4FA013);兰州市科技计划项目(2020-XG-21)。

摘　　要：与传统的微生物鉴定技术相比,基质辅助激光解吸电离飞行时间质谱(matrix-assisted laser desorption ionization time-of-flight mass spectrometry, MALDI-TOF MS)是一种准确、可靠和快速的鉴定和分型的技术。本文通过检索近年来国内外相关研究论文,总结最新的研究进展,发现MALDI-TOF MS在临床病原微生物、食源性微生物以及环境微生物等鉴定中有较大的优势,加快了微生物鉴定的进程,同时探索该技术在新领域的最新进展和面临的挑战,以期为我国基质辅助激光解吸电离飞行时间质谱技术的发展提供参考。Compared with traditional techniques for microbial identification,matrix-assisted laser desorption ionization time-of-flight mass spectrometry(MALDI-TOF MS)is an accurate,reliable,and rapid technology for identification and typing.We reviewed the relevant research papers in recent years and summarized the latest research progress.MALDI-TOF MS has great advantages in the identification of clinical pathogenic microorganisms,foodborne microorganisms,and environmental microorganisms,which accelerates the process of microbial identification.Furthermore,we explored the latest progress and challenges of this technology in new fields,aiming to provide reference for the development of MALDI-TOF MS in China.

关 键 词：基质辅助激光解吸电离飞行时间质谱 微生物 鉴定 

分 类 号：O657.63[理学—分析化学] R446.5[理学—化学]