血清4型禽腺病毒广西分离株单克隆抗体的制备及鉴定  

Preparation of the Monoclonal Antibody against Serotype 4 Fowl Adenovirus Guangxi Isolate

作　　者：谢丽基 邓显文 谢芝勋 韦悠 谢志勤 罗思思 李孟 黄娇玲 张民秀 范晴 王盛 曾婷婷 张艳芳 XIE Li-ji;DENG Xian-wen;XIE Zhi-xun;WEI You;XIE Zhi-qin;LUO Si-si;LI Meng;HUANG Jiao-ling;ZHANG Min-xiu;FAN Qing;WANG Sheng;ZENG Ting-ting;ZHANG Yan-fang(Guangxi Veterinary Research Institute,Guangxi Key Laboratory of Veterinary Biotechnology,Key Laboratory of China(Guangxi)-ASEAN Cross-borderAnimal Disease Prevention and Control,Ministry of Agriculture and Rural Affairs of China,Nanning 530001,China)

机构地区：[1]广西壮族自治区兽医研究所,广西兽医生物技术重点实验室,农业农村部中国(广西)-东盟跨境动物疫病防控重点实验室,南宁530001

出　　处：《中国兽药杂志》2023年第10期9-15,共7页Chinese Journal of Veterinary Drug

基　　金：广西青年科学基金项目(2020GXNSFBA297104);广西人才专项(桂科AD17195083);广西八桂学者专项(2019A50)。

摘　　要：为制备血清4型禽腺病毒(FAdV-4)广西分离株(FAdV-4-GX2018-005)的特异性单克隆抗体,将FAdV-4-GX2018-005作为免疫原免疫BALB/c小鼠后,取小鼠脾细胞与SP2/0细胞融合,利用间接ELISA法筛选阳性杂交瘤细胞株。利用阳性杂交瘤细胞株制备腹水并纯化得到单克隆抗体,利用ELISA和IFA鉴定单克隆抗体的特异性。结果表明,成功获得3株单克隆细胞株3A3、13A11和4D5。3株杂交瘤细胞株分泌的抗体,能与FAdV-4产生特异性反应,与其他血清型禽腺病毒(FAdV-1、FAdV-2、FAdV-3、FAdV-5、FAdV-6、FAdV-7、FAdV-8a、FAdV-8b、FAdV-9、FAdV-10和FAdV-11)不发生反应。本研究成功制备FAdV-4广西分离株单克隆抗体,为FAdV-4检测方法的建立奠定了良好基础。To prepare monoclonal antibodies specific to fowl adenovirus serotype 4(FAdV-4)Guangxi isolate(FAdV-4-GX2018-005),this study used FAdV-4-GX2018-005 as an immunogen to immunize BALB/c mice,splenocytes were harvested and fused with SP2/0 cells.Positive cell lines were identified by ELISA.Ascites were prepared from positive cell line and purified.The specificity of the monoclonal antibody was determined by ELISA and IFA.Three hybridomas(3A3,13A11 and 4D5)secreting monoclonal antibodies against FAdV-4 were selected.ELISA and IFA results showed that those monoclonal antibodies could react specifically with FAdV-4 virus,and react negative with other serotypes of fowl adenovirus(FAdV-1,FAdV-2,FAdV-3,FAdV-5,FAdV-6,FAdV-7,FAdV-8a,FAdV-8b,FAdV-9,FAdV-10 and FAdV-11).The successful development of monoclonal antibody for FAdV-4-GX2018-005 has laid a good foundation for the development of FAdV-4 detection method.

关 键 词：血清4型禽腺病毒 广西分离株 单克隆抗体 

分 类 号：S852.65[农业科学—基础兽医学]