鸽新城疫rGX-mF株灭活疫苗的制备及免疫原性分析  

Preparation and Immunogenicity Analysis of Inactivated Vaccine of Pigeon Newcastle Disease rGX-mF Strain

作　　者：涂敏 赵蕾[1] 史爱华[1] 沈佳[1] 习硕 张建伟[1] 彭湃 王海良 章振华[1] 靳换 TU Min;ZHAO Lei;SHI Ai-hua;SHEN Jia;XI Shuo;ZHANG Jian-wei;PENG Pai;WANG Hai-liang;ZHANG Zhen-hua;JIN Huan(Institute of Animal Husbandry and Veterinary Medicine,Beijing Academy of Agriculture and Forestry Sciences;Beijing Lingyu Biotechnology Co.,Ltd.;Beijing Veterinary Medicine and Feed Monitoring Center)

机构地区：[1]北京市农林科学院畜牧兽医研究所,北京100097 [2]北京翎羽生物科技有限公司,北京101302 [3]北京市兽药饲料检测中心,北京102200

出　　处：《中国兽药杂志》2023年第10期25-32,共8页Chinese Journal of Veterinary Drug

基　　金：北京市农林科学院青年基金(QNJJ202111);北京市农林科学院创新能力建设(KJCX20230424);北京市农林科学院畜牧兽医研究所改革与发展(XMS202313);国家自然科学基金青年基金(32202793)。

摘　　要：为有效控制临床中鸽新城疫的发生,选取基因Ⅵ型鸽新城疫病毒弱毒株rGX-mF作为疫苗候选株,评价其生物学特性,并将rGX-mF灭活后制备成油佐剂灭活疫苗,接种雏鸽和SPF鸡进行rGX-mF株的免疫原性分析,为研制安全高效的鸽新城疫灭活疫苗奠定基础。结果表明,rGX-mF株能够在鸡胚中稳定传代,效价高,毒力弱;rGX-mF株灭活疫苗接种雏鸽后疫苗吸收良好,无局部和全身不良反应,安全性好,免疫持续期长达12个月;rGX-mF株灭活疫苗与LaSota株灭活疫苗相比,rGX-mF株灭活疫苗在鸽体内能产生更高水平的抗体;rGX-mF株灭活疫苗同时免疫SPF鸡和雏鸽,SPF鸡产生的抗体显著高于鸽产生的抗体,这可能与鸽和鸡的免疫系统存在差异有关。综上,利用基因Ⅵ型鸽新城疫病毒弱毒rGX-mF株制备的灭活疫苗可在鸽体内产生有效的抗体,免疫效果好,免疫持续期长。The attenuated strain rGX-mF of gene Ⅵ type pigeon newcastle disease virus was selected as a vaccine candidate,and its biological characteristics were evaluated.Pigeons and SPF chickens were inoculated to analyze the immunogenicity of rGX-mF strain.The rGX-mF strain was inactivated,and an oil adjuvant inactivated vaccine was prepared,which was used to control the occurrence of pigeon newcastle disease.These will laying the foundation for the development of a safe and efficient pigeon newcastle disease inactivated vaccine.The results showed that the rGX-mF strain can be stably passaged in chicken embryos with high potency and weak toxicity;The rGX-mF inactivated vaccine is well absorbed in pigeons after inoculation.The immunity period of the vaccine is up to 12 months;When compared with the La Sota strain,the rGX-mF strain induces higher levels of antibodies in pigeons;The SPF chickens and pigeons were immunized with rGX-mF inactivated vaccine at the same time,and the antibody level of SPF chickens was significantly higher than that of pigeons,which is related to the differences of immune systems between pigeons and chickens.In summary,the rGX-mF inactivated vaccine,an attenuated strain of gene Ⅵ type pigeon newcastle disease virus,can produce effective antibodies in pigeons.

关 键 词：基因Ⅵ型 鸽新城疫 灭活疫苗 免疫原 抗体 

分 类 号：S859.79[农业科学—临床兽医学]