UPLC-PDA联合UPLC-MS/MS法确证一批标称杨树花口服液中非法添加物甘草酸  

UPLC-PDA Combined UPLC-MS/MS Method to Confirm the Illegal Addition of Glycyrrhizic Acid in One Batch of Nominal Yangshuhua Koufuye

作　　者：魏秀丽 张传津 刘道中 李有志 杨志昆 陈志强 WEI Xiu-Li;ZHANG Chuan-Jin;LIU Dao-zhong;LI You-Zhi;YANG Zhi-Kun;CHEN Zhi-qiang(Shandong Center for Quality Control of Feed and Veterinary Drugs;Shandong Provincial Key Laboratory of Quality Safty Monitoring and Risk Assessment for Animal Products;China Shandong Engineering Laboratory for Drug Resistance Monitoring and Precise Drug Use of Animal Origin Bacteria,Jinan 250022,China)

机构地区：[1]山东省饲料兽药质量检验中心 [2]山东省畜产品质量安全监测与风险评估重点实验室 [3]动物源细菌耐药性监测与精准化用药山东省工程实验室,济南250022

出　　处：《中国兽药杂志》2023年第10期47-53,共7页Chinese Journal of Veterinary Drug

基　　金：2019年山东省农业应用重大创新专项“蛋鸡安全生产关键技术集成创新与示范”(SD2019XM004);李有志泰山产业领军人才项目(TSCX202211023)。

摘　　要：采用UPLC-PDA联合UPLC-MS/MS方法确证了一批标称杨树花口服液中非法添加物甘草酸。样品经提取稀释后,采用ACQUITY UPLC T3色谱柱为分离柱,UPLC-PDA初步筛查,再用负离子扫描,液相色谱串联质谱仪上机测定。通过对比色谱图保留时间和光谱图的峰形以及质谱图,结果表明最终确证该批样品中非法添加物为甘草酸。UPLC-PDA方法中甘草酸检测限为20μg/mL,定量限为50μg/mL。本方法快速、灵敏、重现性好,适用于本批标称杨树花口服液中甘草酸的检测。The illegal addition of glycyrrhizic acid in a batch of nominal Yangshuhua Koufuye was confirmed using UPLC-PDA combined with UPLC-MS/MS method.After extraction and dilution,ACQUITY UPLC T3 column was used as separation column,UPLC-PDA,and liquid chromatography tandem mass spectrometer.By comparing the retention time of the chromatogram and the peak shape of the spectrogram and the mass spectrometer,the results showed that the illegal addition was glycyrrhizic acid.For UPLC-PDA,the limit of detection was 20μg/mL and the limit of quantification was 50μg/mL.This method is fast,sensitive and reproducible,and is suitable for the detection of glycyrrhizic acid in this nominal Yangshuhua koufuye.

关 键 词：杨树花口服液 甘草酸 非法添加 

分 类 号：S859.79[农业科学—临床兽医学]