基于特征图谱和网络药理学的葛根芩连汤树脂醇提物质量标志物(Q-marker)预测  

Q-marker prediction of resin ethanol extract of Gegen Qinlian Decoction based on characteristic spectrum and network pharmacology

作　　者：杨晓琴 吴淑洋 李敏[1] 陈佳美 成颜芬 王一涛[1,2] 吴亿晗 章津铭[1] YANG Xiao-qin;WU Shu-yang;LI Min;CHEN Jia-mei;CHENG Yan-fen;WANG Yi-tao;WU Yi-han;ZHANG Jin-ming(State Key Laboratory of Southwestern Chinese Medicine Resources,School of Pharmacy,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China;State Key Laboratory of Quality Research in Traditional Chinese Medicine,Institute of Chinese Medical Sciences,University of Macao,Macao 999078,China)

机构地区：[1]成都中医药大学药学院西南特色中药资源国家重点实验室,四川成都611137 [2]澳门大学中华医药研究院中药质量研究国家重点实验室,中国澳门999078

出　　处：《中国中药杂志》2023年第18期4993-5002,共10页China Journal of Chinese Materia Medica

基　　金：国家中医药管理局中医药创新团队及人才支持计划项目(ZYYCXTD-D-202209)。

摘　　要：葛根芩连汤树脂醇提物可显著缓解伊立替康所致的肠毒性,但其整体质量和临床用药标准有待进一步研究。该文旨在建立葛根芩连汤树脂醇提物HPLC特征图谱,联合网络药理学预测作用靶点和信号通路的方法,预测出药效相关性的核心化合物,分析预测葛根芩连汤树脂洗脱物缓解伊立替康的潜在的质量标志物(Q-marker)。根据质量标志物“五原则”的特有性、可测性和溯源性,结合网络药理学技术可表征葛根芩连汤树脂醇提物的整体功效,初步预测葛根素、小檗碱、黄芩苷、黄芩素4个成分为葛根芩连汤树脂醇提物潜在的Q-marker,为葛根芩连汤树脂醇提物的质量控制和临床作用机制提供依据和参考。The resin ethanol extract of Gegen Qinlian Decoction(GGQLD)has been found to significantly alleviate the intestinal toxicity caused by Irinotecan,but further research is needed to establish its overall quality and clinical medication standards.This study aimed to establish an HPLC characteristic fingerprint of the resin ethanol extract of GGQLD,predicted the targets and signaling path-ways of its pharmacological effects based on network pharmacology,identified core compounds with pharmacological relevance,and analyzed potential quality markers(Q-markers)of the resin eluate of GGQLD for relieving Irinotecan-induced toxicity.By considering the uniqueness,measurability,and traceability of Q-markers based on the"five principles"of Q-markers and combining them with net-work pharmacology techniques,the overall efficacy of the resin ethanol extract of GGQLD can be characterized.Preliminary predictions suggested that the four components of puerarin,berberine,baicalin,and baicalein might serve as potential Q-markers for the resin etha-nol extract of GGQLD.This study provides a basis and references for the quality control and clinical mechanism of the resin ethanol ex-tract of GGQLD.

关 键 词：葛根芩连汤 大孔树脂醇提物 特征图谱 网络药理学 质量标志物 

分 类 号：R285[医药卫生—中药学]