超高效液相色谱-串联质谱法测定饲料中的喹烯酮  

Determination of quinocetone in feed by ultra performance liquid chromatography-tandem mass spectrometry

作　　者：王鑫 杨发树 杜雪莉 张凤枰 WANG Xin;YANG Fashu;DU Xueli;ZHANG Fengping(Sichuan Willtest Technology Co.Ltd.,Chengdu,Sichuan Province 610041,China;Key Laboratory of Aquatic,Livestock,Poultry Nutrition and Healthy Culturing,Ministry of Agriculture and Rural Affairs,Key Laboratory of Aquatic Healthy Aquaculture of Sichuan Province,Tongwei Co.Ltd.,Chengdu,Sichuan Province 610093,China)

机构地区：[1]四川威尔检测技术股份有限公司,四川成都610041 [2]通威股份有限公司,农业农村部水产畜禽营养与健康养殖重点实验室,四川省水产健康养殖重点实验室,四川成都610093

出　　处：《中国饲料》2023年第21期143-147,共5页China Feed

基　　金：国家标准化管理委员会2020年第三批推荐性国家标准计划(20203890-T-469);四川省青年科技创新研究团队专项计划项目(2019JDTD0025)。

摘　　要：本研究采用超高效液相色谱-串联质谱法测定饲料中喹烯酮的残留量。样品经乙腈-乙酸乙酯(1:1,V/V)提取、正己烷除脂净化后,用乙腈-0.2%甲酸作为流动相,经C_(18)分离、LC-MS/MS多反应监测、正离子模式分析,基质匹配外标法定量。结果表明:在浓度为1.035~103.5 ng/mL,四种饲料喹烯酮基质匹配标准工作曲线线性关系良好,相关系数均在0.998以上;方法检出限为6.0μg/kg、定量限为20.0μg/kg,方法加标回收率为89.6%~116.0%、相对标准偏差为3.44%~7.19%,适用于配合饲料、浓缩饲料、精料补充料和添加剂预混合饲料中喹烯酮残留量的检测。In this study,ultra performance liquid chromatography tandem mass spectrometry was used to determine the residues of quinocetone in feed.The samples were extracted with acetonitrile-ethyl acetate(1:1,V/V),and purified by n-hexane.Acetonitrile-0.2%formic acid was used as the mobile phase,and multiple reaction monitoring and positive ion mode were used for qualitative detection and quantitative analysis.The results showed that the linear relationship of quinocetone was good,the correlation coefficients were all above 0.998,the detection limit of the method was 6.0μg/kg,and the limit of quantification was 20.0μg/kg,the average recovery at different levels was 89.6%~116.0%,with the relative standard deviation was 3.44%~7.19%.The method had good repeatability and could be applied to detection of quinocetone residues in feed.

关 键 词：喹烯酮 超高效液相色谱-串联质谱法 饲料 

分 类 号：S816.17[农业科学—饲料科学]