牛分枝杆菌CVCC68002全基因组测序与功能分析  

Whole genome sequencing and functional analysis of Mycobacterium bovis CVCC68002

作　　者：王霞 马伟超 雒林通 裴芳 王廷璞 WANG Xia;MA Weichao;LUO Lintong;PEI Fang;WANG Tingpu(College of Bioengineering and Technology,Tianshui Normal University,Tianshui 741001,China;Laboratory Medicine,Tianshui Fifth People´s Hospital,Tianshui 741024,China)

机构地区：[1]天水师范学院生物工程与技术学院,甘肃天水741001 [2]天水市第五人民医院检验科,甘肃天水741024

出　　处：《甘肃农业大学学报》2023年第5期11-24,共14页Journal of Gansu Agricultural University

基　　金：国家自然科学基金项目(31860707);甘肃省自然科学基金项目(20JR10RA796);甘肃省高等学校创新能力提升项目(2020B-171)。

摘　　要：【目的】为进一步深入研究牛分枝杆菌CVCC68002基因组特征,致病性和遗传进化关系,为牛分支杆菌感染疾病的预防和深入研究提供参考。【方法】利用Illumina和Pacbio测序技术平台对CVCC68002菌株进行全基因组测序建库,采用生物信息学和系统进化树等方法,对该菌株基因组功能注释、致病性和遗传进化关系进行了分析。【结果】通过分析发现该菌株基因组全长4357100 bp,GC含量为65.64%,编码基因达4145个;在GO、KEGG、COG、NR数据库基因组特征显示其具有高氨基酸和碳水化合物代谢能力。通过PHI,VFDB,ARDC和CARD数据库对其致病性进行相关分析,得到456个毒力基因注释,主要涉及细菌黏附与定殖、细菌逃避宿主免疫防御系统、分泌系统蛋白和转录因子等。并找到相关包括万古霉素,青霉素,多胺类抗生素、异烟肼、吡嗪酰胺、氟喹诺酮等的抗药性基因,另外,外排泵的抗性蛋白注释量显著,推测牛分枝杆菌通过外排抗性蛋白从而达到抗药自我保护的目的。【结论】获得牛分枝杆菌CVCC68002株完整基因组信息,完善系统进化信息,明确了其毒力因子和有效地靶标抗药基因,为后续对该菌株分子生物学特征、致病性和耐药基因筛选研究奠定了理论基础。【Objective】The study aimed to study genomic characteristics,pathogenicity and genetic evolution of Mycobacterium bovis CVCC68002,providing reference to the prevention and in-depth study of infectious diseases of Mycobacterium bovis.【Method】The whole genome of CVCC68002 strain was sequenced by using Illumina and pacbio sequencing technology platforms,followed by establishing the library.The genomic function annotation analysis,pathogenicity and genetic evolution relationship analysis of the strain were performed by using bioinformatics and phylogenetic number.【Result】The total genome length of the strain was 4357100 bp,with 65.64%of GC,and there were 4145 coding genes.The genomic characteristics of GO,KEGG,COG and NR databases showed that they had the functions in high amino acid and carbohydrate metabolism.The pathogenicity was analyzed through the databases of PHI,VFDB,ARDC and CARD,and the 456 virulence gene annotations were obtained,which were mainly related to bacterial adhesion and colonization,bacterial escape from the host immune defense system,secretory system proteins and transcription factors.The relevant drug-resistance genes,including vancomycin,penicillin,polyamine antibiotics,isoniazid,pyrazinamide,fluoroquinolone,etc.,were found by ARDB and CARD databases.In addition,the annotation amount of resistance protein of efflux pump was significant,and it was anticipated the bacteria could protect themselves by efflux of resistance protein.【Conclusion】The complete genome information of Mycobacterium bovis CVCC68002 strain was obtained,and the phylogenetic information was improved.The virulence factors and effective target drug resistance genes were identified,which laid a theoretical foundation for the further study on molecular biological characteristics,pathogenicity and drug resistance gene screening of the strain.

关 键 词：牛分枝杆菌 基因组测序 致病性分析 毒力因子 耐药性分析 

分 类 号：Q93[生物学—微生物学]